调心安神针法对缺血再灌注心律失常大鼠心肌组织微小RNA的影响

Effect of Tiaoxin Anshen Acupuncture on MicroRNAs of Myocardial Tissue of Ischemia-Reperfusion Arrhythmic Rats

目的观察调心安神针法对缺血再灌注心律失常(RA)大鼠心肌组织微小RNA(miRNA1、miRNA133a)表达的影响以了解调心安神针法治疗缺血再灌注心律失常的作用机制。方法40只大鼠随机分为假手术组、模型组、针刺组及胺碘酮组,针刺组施以调心安神针刺法预处理7 d(取穴:灵台、神道、双侧内关、百会)。采用结扎/松解左冠状动脉前降支的方法制作RA模型大鼠。造模完毕后,酶联免疫法检测血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)含量,苏木精-伊红染色观察心肌组织,RT-PCR法检测缺血区中央部位心内膜心肌miRNA1、miRNA133a的表达水平。结果针刺组、胺碘酮组CK-MB和LDH的含量较模型组明显降低(P<0.01)。针刺组、胺碘酮组miRNA1表达均较模型组明显降低(P<0.01),且胺碘酮组低于针刺组(P<0.01);针刺组和胺碘酮组miRNA133a表达均较模型组明显增加(P<0.05),针刺组与胺磺酮组差异无统计学意义(P>0.05)。结论调心安神针法预处理和注射胺碘酮均能改善心肌损伤,减少心律失常的发生,其机制可能与通过下调缺血再灌注心律失常大鼠心肌组织miRNA1表达,上调miRNA133a的表达有关。

Objective:To observe the effect of Tiaoxin Anshen acupuncture on the expression of miRNA(miRNA1,miRNA133a)in myocardium of ischemia reperfusion arrhythmia(RA)model rats,and to explore the mechanism of ischemia reperfusion arrhythmia.Methods:RA model rats were made by ligating and loosening the anterior descending branch of the left coronary artery.40 Rats were divided into sham operation group,model group,acupuncture group and amiodarone group.The acupuncture group were given Tiaoxin Anshen acupuncture pretreatment for 7 days at Lingtai(GV10),Shendao(GV11),bilateral Neiguan(PC6)and Baihui(GV20).After the experiment,ELISA method was used to detect serum CK-MB and LDH content,and HE staining was used to observe myocardial tissue,RT-PCR method was used to detect the expression levels of miRNA1 and miRNA133a in the endocardial myocardium in the central part of the ischemic area.Results:The contents of CK-MB and LDH in acupuncture group and amiodarone group were significantly lower than those in model group(P<0.01).The expression of miRNA1 in acupuncture group and amiodarone group was significantly lower than that in model group(P<0.01),and amiodarone group was lower than that in acupuncture group(P<0.01).The expression of miRNA133a in acupuncture group and amiodarone group was significantly increased compared with model group(P<0.05),but there was no difference between the two groups(P>0.05).Conclusion:Tiaoxin Anshen acupuncture pretreatment and amiodarone injection could improve myocardial damage and reduce the occurrence of ischemiareperfusion arrhythmias,and the mechanism may be related to the downregulation of miRNA1 and upregulation of miRNA133a in myocardial tissue of ischemia-reperfusion rats.