细粒棘球绦虫胞外囊复合物2蛋白的生物信息学分析

Bioinformatics Analysis of the Extracellular Vesicle Complex 2 Protein of the Cestode Echinococcus Granulosus

目的:利用生物信息学方法预测细粒棘球绦虫胞外囊复合物2(EXOC2)蛋白的生化特性、抗原表位及分子功能。方法:利用蛋白质在线分析网站以及在线数据库预测EXOC2蛋白的理化性质、三级结构、亲疏水性、B细胞优势抗原表位、T细胞优势表位、蛋白的互作网络关系、差异基因GO功能、KEGG通路富集分析、磷酸化位点和蛋白修饰位点。结果:优势B细胞表位位于100-115、539-540、851-866、896-911、988-992、704-749区段,HLA-DRB1*0401限制性Th表位位于821-823、920-930区段,HLA-DRB1*0701限制性Th表位位于270-275、920-922区段。结论:利用在线数据库及生物信息学方法预测EXOC2蛋白的生物功能、B细胞优势抗原表位及T细胞优势,可为棘球蚴病分子肽疫苗的研制提供一定的理论基础。

Objective:To predict the biochemical properties,antigenic epitopes,and molecular functions of the extracellular vesicle complex 2(EXOC2)protein of the fine-grained echinococcus tapeworm,Echinococcus granulosus and bioinformatics methods,etc.Methods:The physicochemical properties,tertiary structure,hydrophilicity,B-cell dominant antigenic epitopes,T-cell dominant epitopes,protein interactions,differential gene GO function and KEGG pathway enrichment analysis,phosphorylation sites,and protein modification sites of EXOC2 proteins were predicted by using the Protein Analytics Online website as well as an online database.Results:The dominant B-cell epitopes were located at 100-115,539-540,851-866,896-911,988-992,704-749,the HLA-DRB1*0401-restricted Th epitopes were located at 821-823,920-930,and the HLA-DRB1*0701-restricted Th epitopes were located at 270-275,920-922 compartments.Conclusion:The biological functions and dominant T and B cell antigenic epitopes of the EXOC2 protein were predicted using online databases and bioinformatics methods,which may provide some theoretical basis for the development of molecular peptide vaccines against echinococcosis.