猪细小病毒血凝抑制试验抗原、阳性血清与阴性血清的制备与鉴定

Preparation and Identification of Porcine Parvovirus Hemagglutination Inhibition Test Antigen,Positive Serum and Negative Serum

猪细小病毒(Porcine parvovirus,PPV)血凝抑制试验抗原、阳性血清和阴性血清在猪细小病毒制品的效力检验中不可或缺,对猪细小病毒相关生物制品的质量控制至关重要。试验采用PPV 7909株病毒同步接种PK-15细胞制备血凝抑制试验抗原,用制备的抗原乳化后免疫豚鼠制备阳性血清,同时用未免疫的阴性豚鼠制备阴性血清。对抗原、阳性血清和阴性血清进行鉴定,结果表明,制备的PPV血凝抑制试验抗原HA效价达1∶512;阳性血清HI效价达1∶1024;阴性血清HI效价小于1∶8,且特异性均良好。用制备的HI试验抗原与不同PPV疫苗株的阳性血清进行HI试验,同时,用制备的阳性血清、阴性血清与不同PPV疫苗株的灭活抗原进行HI试验。结果表明,制备的抗原与阳性血清具备良好的血清学交叉反应性,制备的阴性血清与不同疫苗株抗原均呈阴性反应,可用于PPV制品的统一评价。

Porcine parvovirus(PPV)hemagglutination inhibition test antigen,positive serum and negative serum are indispensable for the efficacy test of PPV products,and it is very important for the quality control of PPV related biological products.The hemagglutination inhibition test antigen was prepared by simultaneous inoculation of PPV 7909 strain with PK-15 cells.The positive serum was prepared by emulsifying the antigens and immunizing guinea pigs,and the negative serum was prepared by non-immunized negative guinea pigs.Antigen,positive serum and negative serum are identified,the results show that the PPV hemagglutination inhibition test antigen HA titer was 1∶512;the positive serum HI titer was 1∶1024;and the negative serum HI titer<1∶8,all of the preparation have good specificity.Cross-reaction test was conducted by using the prepared hemagglutination inhibition test antigen,positive serum and the inactivated antigen,positive serum of different PPV vaccine strains,the results showed that the antigen and positive serum had good serological cross-reaction and could be used for unified evaluation of PPV products.