金黄色葡萄球菌感染对特应性皮炎患者色氨酸代谢的影响

Effect of Staphylococcus aureus infection on tryptophan metabolism in patients with atopic dermatitis

目的探讨金黄色葡萄球菌感染对特应性皮炎患者体内色氨酸代谢的影响。方法纳入2020年1月-2023年1月中国人民解放军海军第九七一医院收治的特应性皮炎患者106例和健康受试者89名。采集特应性皮炎患者的皮肤样本,病变样本为病变组,非病变样本为非病变组,健康受试者皮肤样本为对照组。检测3组样本中金黄色葡萄球菌的载量、金黄色葡萄球菌CC1株和CC30株的比例、皮肤屏障理化性质、色氨酸代谢产物水平[吲哚-3-丙烯酸(IA)、吲哚乙酸(IAA)、吲哚-3-乳酸(ILA)、吲哚-3-丙酸(IPA)、吲哚-3-甲醇(I3C)、吲哚-3-甲醛(IAId)、L-色氨酸(Trp)、5-羟色氨酸(5-HTP)、L-犬尿氨酸(KYN)]。结果相比于对照组和非病变组,病变组皮肤金黄色葡萄球菌的载量较高、CC1株比例较高、CC30株比例较低(P<0.05),皮肤屏障理化性质下降(P<0.05),色氨酸代谢产物IA、IAA的水平升高(P<0.05),色氨酸代谢产物ILA、IPA、IAId的水平下降(P<0.05);金黄色葡萄球菌的载量、CC1株比例和CC30株比例均与皮肤屏障理化性质存在相关性;金黄色葡萄球菌的载量、CC1株比例和CC30株比例均与色氨酸代谢产物IA、IAA、ILA、IPA、IAId存在相关性;皮肤屏障理化性质均与色氨酸代谢产物IA、IAA、ILA、IPA、IAId存在相关性。结论特异性皮炎患者皮肤金黄色葡萄球菌的载量较高,金黄色葡萄球菌水平与皮肤屏障理化性质、色氨酸代谢物产物IA、IAA、ILA、IPA、IAId存在相关性。

OBJECTIVE To investigate the effects of Staphylococcus aureus infection on tryptophan metabolism in patients with atopic dermatitis.METHODS A total of 106 patients with atopic dermatitis and 89 healthy volunteers admitted to the 971st Hospital of the Chinese People's Liberation Army Navy from Jan 2020 to Jan 2023 were re-cruited.The skin samples of atopic dermatitis patients were collected.The pathological samples were in the patho-logical group and the non-pathological samples were in the non-pathological group.Skin samples from healthy sub-jects were the control group.Staphylococcus aureus load,proportions of Staphylococcus aureus CC1 strain and CC30 strain,physical and chemical properties of skin barrier,level of tryptophan metabolates,including indole-3-acetic acid(IA),indole-acetic acid(IAA),and indole-in 3 groups of samples were detected 3-lactate(ILA),in-dole-3-propionic acid(IPA),indole-3-aldehyde(I3C),indole-3-formaldehyde(IAId),L-tryptophan(Trp),5-hydroxytryptophan(5-HTP)and L-kynurenine(KYN).RESULTS Compared with the control group and the non-pathological group,the load of Staphylococcus aureus and the proportion of CC1 strain in the pathological group were higher,while the proportion of CC30 strain was lower(P<0.05);the physicochemical properties of the skin barrier were decreased(P<0.05);the levels of tryptophan metabolites IA and IAA were increased(P<0.05).The levels of tryptophan metabolites such as ILA,IPA and IAId were decreased(P<0.05).The load of Staphylococcus aureus,the proportions of CC1 and CC30 were correlated with the physicochemical properties of the skin barrier.Staphylococcus aureus load,proportions of CC1 strain and CC30 strain were correlated with tryp-tophan metabolites,including IA,IAA,ILA,IPA and IAId.The physical and chemical properties of skin barrier were correlated with tryptophan metabolism products of IA,IAA,ILA,IPA and IAId.CONCLUSION The load of Staphylococcus aureus in the skin of patients with specific dermatitis is higher,and the level of Staphylococcus aureus is correlated with the physicochemical properties of the skin barrier,tryptophan metabolites IA,IAA,ILA,IPA and IAId.