根皮素对肺癌细胞增殖与凋亡的影响

Effect of Proliferation and Apoptosis of Phloretin on the Lung Cancer Cells

以人肺癌细胞A549为研究对象,探讨了根皮素对肺癌细胞增殖和凋亡的影响作用。以不同含量的根皮素处理A549细胞,分析检测细胞毒性、形态学、细胞凋亡率、细胞周期、线粒体膜电位和胞内钙离子稳态。结果表明:根皮素抑制了细胞增殖并使其出现凋亡形态学改变,凋亡率均呈浓度与时间依赖性;细胞被阻滞于细胞周期的G1期,细胞线粒体膜电位降低,胞内游离钙离子含量增加,钙离子稳态失调。A549细胞周期、线粒体功能及胞内钙离子稳态均受到根皮素干扰。

The effect of phloretin on the proliferation and apoptosis of human lung cancer cell line A549 was investigated.A549 cells were treated with different concentrations of phloretin,and the cytotoxicity,morphology,apoptosis rate,cell cycle,mitochondrial membrane potential and intracellular calcium homeostasis were analyzed.The results showed that phloretin inhibited cell proliferation and induced morphological changes of apoptosis in a concentration and time dependent manner.The cells were arrested in the G1 phase of the cell cycle,the mitochondrial membrane potential was decreased,the intracellular free calcium ion content was increased,and the calcium ion homeostasis was disturbed.Phloretin disrupted the cell cycle,mitochondrial function and intracellular calcium homeostasis in A549 cells.