MSTN^(-/-)鲁西黄牛肌肉全转录组共表达网络及转录本富集分析

Whole transcriptome coexpression network and transcript enrichment analysis in the muscle of MSTN^(-/-)Luxi cattle

为了探究与牛骨骼肌发育相关的共表达网络及核心转录本,挖掘牛骨骼肌在发育过程中的调控转录本,试验采用新的fastp+kallisto+Sleuth分析方法对牛骨骼肌转录组测序数据进行分析,通过转录组测序分析筛选出两组肌肉样本差异表达的RNA,对其进行GO功能注释分析和KEGG信号通路富集分析、WGCNA分析和GSEA富集分析。结果表明:转录组测序共鉴定出32919个转录本,按照是否满足|lbFC|≥2、P<0.05的条件共筛选出457个差异表达转录本,其中上调转录本303个,下调转录本154个。457个差异表达转录本共涉及106个GO功能注释条目和14个KEGG信号通路,差异表达转录本主要富集在与细胞周期、细胞生长密切相关的基因功能注释和信号通路中。457个差异表达转录本被划分到显著相关的3个转录本模块,棕色模块有83个转录本,蓝色模块有93个转录本,绿色模块有111个转录本,并且筛选出10个核心转录本。核心转录本多数集中在细胞黏附分子、AMPK信号通路、PI3K-Akt信号通路、肌动蛋白细胞骨架的调节等与细胞周期、细胞生长密切相关的通路中。说明牛骨骼肌发育受基因转录水平调控的影响。

In order to explore the coexpression network and core transcripts related to the development of bovine skeletal muscle,and explore the regulatory transcripts of bovine skeletal muscle during development,the new fastp+kallisto+Sleuth analysis method was used to analyze the bovine skeletal muscle transcriptome sequencing data,the differentially expressed RNAs in the two groups of muscle samples were screened by transcriptome sequencing analysis,and the GO functional annotation analysis,KEGG signaling pathway enrichment analysis,WGCNA analysis,and GSEA enrichment analysis were performed.The results showed that a total of 32919 transcripts were identified by transcriptome sequencing,and 457 differentially expressed transcripts were screened according to whether they met the conditions of|lbFC|≥2 and P<0.05,including 303 up-regulated transcripts and 154 down regulated transcripts.The 457 differentially expressed transcripts involved a total of 106 GO functional annotation entries and 14 KEGG signaling pathways.The differentially expressed transcripts were mainly enriched in gene function annotation and signaling pathways closely related to cell cycle and cell growth.The 457 differentially expressed transcripts were divided into three significantly related transcript modules,including 83 transcripts in brown module,93 transcripts in blue module and 111 transcripts in green module,and 10 core transcripts were screened out.Most of the core transcripts were concentrated in the pathways closely related to cell cycle and cell growth,such as cell adhesion molecules,MAPK signaling pathway,PI3K-Akt signaling pathway,and the regulation of actin cytoskeleton.These results indicated that the development of bovine skeletal muscle was affected by the regulation of gene transcription level.