iNOS/CD163在人慢性根尖周炎组织中CD68^(+)细胞上的表达

Expression of iNOS/CD163 in CD68^(+)cells from human chronic apical periodotitis tissues

目的:观察诱导型一氧化氮合酶(iNOS)和CD163在人不同类型的慢性根尖周炎组织中CD68^(+)细胞的表达,分析在人不同类型的慢性根尖周炎组织中CD68^(+)细胞的表达情况。方法:45名受试者按根尖周炎类型分为3组(健康对照组、慢性根尖脓肿组和根尖周囊肿组),每组15名。免疫荧光双染色法观察根尖周标本中CD68^(+)-iNOS^(+)和CD68^(+)-CD163^(+)细胞在各组标本中的表达情况,计算双阳性细胞的平均密度。结果:(1)3组标本中CD68^(+)-iNOS^(+)和CD68^(+)-CD163^(+)细胞密度差异显著(P<0.01);(2)慢性根尖脓肿组标本中CD68^(+)-iNOS^(+)细胞密度高于根尖周囊肿组(P<0.01),而两组间CD68^(+)-CD163^(+)细胞密度无显著差异(P>0.05);(3)慢性根尖周脓肿组中CD68^(+)-iNOS^(+)/CD68^(+)-CD163^(+)比值显著高于根尖囊肿组(P<0.01),而慢性根尖周脓肿组和对照组CD68^(+)-iNOS^(+)/CD68^(+)-CD163^(+)比值的无显著差异(P>0.05)。结论:CD68^(+)-iNOS^(+)/CD68^(+)-CD163^(+)在慢性根尖脓肿组和根尖周囊肿组中表达的比值不同,调整其比值可能涉及慢性根尖周炎不同病程的发展。

AIM:To investigate the expression of inducible nitric oxide synthase(iNOS)and CD163 in periapical tissues at varying stages of human chronic apical periodotitis(CAP).METHODS:Forty-five voluntary samples were collected and categorized into three groups based on the degree of CAP:healthy control group(n=15),chronic apical abscess group(n=15),and periapical cyst group(n=15).Double immunofluorescence staining was employed to examine the expression of CD68^(+)-iNOS^(+)and CD68^(+)-CD163^(+)cells in periapical tissues.RESULTS:(1)The densities of CD68^(+)-iNOS^(+)and CD68^(+)-CD163^(+)cells showed significant differences among CAP groups(P<0.01).(2)The density of CD68^(+)-iNOS^(+)cells in chronic apical abscess group was significantly higher than that in periapical cyst group(P<0.01).However,there was no significant difference in the density of CD68^(+)-CD163^(+)cells between chronic apical abscess group and periapical cyst group(P>0.05).(3)The ratio of CD68^(+)-iNOS^(+)/CD68^(+)-CD163^(+)was significantly higher in chronic apical abscess group compared with periapical cyst group(P<0.01),while there was no significant difference in this ratio between chronic apical abscess group and healthy control group(P>0.05).CONCLUSION:The expression ratio of CD68^(+)-iNOS^(+)/CD68^(+)-CD163^(+)between chronic apical abscess and periapical cyst groups differed.Modulating the proportion of CD68^(+)-iNOS^(+)/CD68^(+)-CD163^(+)may be involved in the development of CAP at various stages.