去铁胺局部注射对小鼠牙槽骨骨改建的实验研究

Local injection of DFO on bone remodeling of alveolar bone:An experimental study in the mouse

目的:探究局部注射去铁胺(desferrioxamine,DFO)通过低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)调控破骨细胞对牙槽骨骨改建的影响。方法:对C57小鼠局部牙槽骨注射DFO,通过micro-CT及组织学染色法观察牙槽骨骨量的变化;通过免疫荧光及抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphase,TRAP)染色观察破骨细胞生成,之后用TRAP、免疫荧光等方法观察体外DFO通过HIF-1α对Raw264.7向破骨细胞分化的影响。结果:Micro-CT及组织学染色显示,DFO局部注射后局部骨密度降低、骨量减少;组织学染色发现,相比对照组,DFO注射组局部破骨细胞数量增多、HIF-1α表达增加;体外细胞培养发现DFO可促进Raw264.7细胞中HIF-1α的表达及其向破骨细胞分化。结论:DFO局部注射可促进牙槽骨骨改建及Raw264.7向破骨细胞分化。

Objective:To investigate the effect of local injection of desferrioxamine(DFO)on the bone remodeling of alveolar bone through the regulation of osteoclasts by hypoxia inducible factor-1α(HIF-1α).Methods:DFO was injected locally into the alveolar bone of C57 mice;changes in alveolar bone volume were observed by micro-CT and histological staining;osteoclastogenesis was observed by immunofluorescence and tartrate-resistant acid phosphase(TRAP)staining;the effects of DFO on the differentiation of Raw264.7 to osteoclasts via HIF-1αwere observed in vitro by TRAP staining and immunofluorescence.Results:Micro-CT and histological staining showed that local bone density decreased and bone volume decreased after local injection of DFO;histological staining revealed that the number of local osteoclasts increased and HIF-1αexpression increased in the DFO injection group compared with the control group;in vitro cell culture revealed that DFO promoted the expression of HIF-1αin Raw264.7 cells and its differentiation to osteoclasts.Conclusion:Local injection of DFO can promote bone remodeling of alveolar bone and differentiation of Raw264.7 into osteoclasts.