酶解豆粕对肉鸡生长性能、抗氧化能力、免疫性能和肠道屏障功能的影响

Effects of enzymolytic soybean meal on growth performance,antioxidant capacity,immunity,and intestinal barrier function of broilers

试验旨在研究酶解豆粕对肉鸡生长性能、抗氧化能力、免疫性能和肠道屏障功能的影响。选取400只1日龄健康的科宝肉鸡,随机分为5个组,每组8个重复,每个重复10只。对照组饲喂基础饲粮,试验组(T1组、T2组、T3组和T4组)饲喂分别用1%、2%、3%和4%酶解豆粕替代基础饲粮中等量豆粕的试验饲粮。试验期42 d。结果表明,与对照组相比,试验组肉鸡的平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G)均无显著差异(P>0.05)。与对照组相比,T3组肉鸡回肠丙二醛(MDA)含量显著降低(P<0.05),T2组和T3组肉鸡肝脏超氧化物歧化酶(SOD)活性显著升高(P<0.05),谷胱甘肽过氧化物酶(GSH-Px)活性显著降低(P<0.05)。与对照组相比,T1组肉鸡肝脏白细胞介素-1β(IL-1β)含量显著降低(P<0.05),T1组和T3组肉鸡肝脏白细胞介素-6(IL-6)含量显著降低(P<0.05)。与对照组相比,试验组肉鸡血清免疫指标均无显著变化(P>0.05)。与对照组相比,T2组和T4组肉鸡血清D-乳酸(D-LA)含量显著降低(P<0.05),T3组的二胺氧化酶(DAO)活性低于对照组(P>0.05)。研究表明,在肉鸡饲粮中添加酶解豆粕可以提高机体抗氧化能力和免疫性能,改善肠道屏障功能,使用3%的酶解豆粕替代肉鸡饲粮中等量的豆粕效果明显。

This experiment was conducted to investigate the effects of enzymolytic soybean meal(ESBM)on the growth performance,antioxidant capacity,immunity,and intestinal barrier function of broilers.A total of 400 healthy one-day-old Cobb broilers were randomly divided into five groups with eight replicates per group and 10 broilers per replicate.The broilers in the control group were fed a basal diet,and the experimental groups(T1 group,T2 group,T3 group,and T4 group)were fed 1%,2%,3%,and 4%ESBM instead of the equivalent amount of soybean meal(SBM)in the basal diet,respectively.The experiment lasted for 42 days.The results showed that compared with the control group,there were no significant differences in the average daily gain(ADG),average daily feed intake(ADFI),and feed to gain ratio(F/G)of broilers in the experimental groups(P>0.05).Compared with the control group,the malondialdehyde(MDA)concentration in the ileum of broilers significantly decreased in the T3 group(P<0.05).The activity of superoxide dismutase(SOD)in the liver significantly increased(P<0.05),and the glutathione peroxidase(GSH-Px)activity significantly decreased in the T2 group and T3 group(P<0.05).Compared with the control group,the interleukin-1β(IL-1β)content in the liver of broilers in the T1 group was significantly decreased(P<0.05),the interleukin-6(IL-6)content in the liver of broilers in the T1 group and T3 group significantly decreased(P<0.05).Compared with the control group,there were no significant effects on the serum immunity indexes of broilers in the experimental groups(P>0.05).Compared with the control group,the D-lactate(D-LA)concentration in the serum of broilers significantly decreased in the T2 group and T4 group(P<0.05),the serum diamine oxidase(DAO)activity in the T3 group was lower than that in the control group(P>0.05).The study indicates that the dietary addition of ESBM can improve the antioxidant capacity and immunity,and improve the intestinal barrier function of broilers.The effect of the equivalent replacement of SBM with 3%ESBM in the diet of broilers is obvious.