不同品种糯红高粱花期内生酵母菌群多样性分析

Diversity of endophytic yeast in different varieties of waxy sorghum at flowering period

采用Illumina MiSeq高通量测序技术对花期糯红高粱不同品种及不同器官的内生酵母菌群多样性进行分析,并采用线性判别分析效应值(LEfSe)及FUNGuild分析样品间的差异菌和功能菌。结果表明,共注释到8门182属。地方种青壳洋内生酵母菌的群落多样性和丰富度均高于杂交种金糯粱1号,叶部丰富度最高。不同品种及茎叶的优势菌门均为子囊菌门(Ascomycota),花和根部的优势菌门分别为担子菌门(Basidiomycota)、未知真菌门(unclassified fungi);在属水平上,金糯粱1号和青壳洋优势菌属分别为刺盾炱目未分类菌属(unclassified Chaetothyriales)和未知真菌属,根、茎、叶和花部的优势菌属分别为未知真菌属、枝氯霉属(Ramichloridium)、刺盾炱目未分类菌属和Didymium。LEfSe分析显示,青壳洋中最重要的差异指示属为Papiliotrema;根、茎、叶和花部最重要的差异指示属分别为镰刀菌属(Fusarium)、枝氯霉属、Alatosessilispora和Pseudozyma。FUNGuild功能预测显示,糯红高粱内生酵母菌群功能丰富,可分为7种营养模式和24个生态功能群,且青壳洋和叶部独有功能菌数最多。

The diversity of endophytic yeast in different varieties and organs of waxy sorghum was analyzed using Illumina MiSeq high-throughput sequencing technology at flowering period,and the differential and functional flora were also analyzed using linear discriminant analysis effect size(LEfSe)and FUNGuild,respectively.The results showed that a total of 182 genera in 8 phyla were annotated.The community diversity and richness of endophytic yeast in Qingkeyang were higher than that of Jinnuoliang 1,and the richness of leaf was the highest.Ascomycota was the dominant phylum in different varieties and the stems and leaves.Basidiomycota was the dominant phylum in flowers,and the dominant phylum in roots was unclassified fungi.At the genus level,the dominant genera in Jinnuoliang 1 and Qingkeyang were unclassified Chaetothyriales and unclassified fungi,respectively.The dominant genera of the roots,stems,leaves and flowers were unclassified fungi,Ramichloridium,unclassified Chaetothyriales and Didymium,respectively.LEfSe analysis showed that the most important difference indicator genus was Papiliotrema.The most important differential indicator genera for roots,stems,leaves and flowers were Fusarium,Ramichloridium,Alatosessilispora and Pseudozyma,respectively.FUNGuild functional prediction showed that waxy sorghum flora has rich functions and was divided into 7 nutritional models and 24 ecological functional groups.Qingkeyang and the leaves had the greatest numbers of unique functional fungus.