大豆DRT100全基因组鉴定及GmDRT100-6响应干旱和盐胁迫的功能分析

Genome-wide Identification of Soybean DRT100 and Function Analysis of GmDRT100-6 Response to Drought and Salt Stress

DNA损伤修复/耐受性100(DNA-damage repair/tolerance 100,DRT100)编码一种富含亮氨酸重复序列的蛋白质,广泛参与植物生长发育与非生物胁迫响应过程。为鉴定大豆(Glycine max L.)中DRT100基因家族成员、揭示其进化关系及其与大豆抗旱耐盐相关功能,本研究在大豆全基因组水平上鉴定出8个DRT100基因,对其基因结构特征、系统进化、启动子顺式作用元件、基因表达模式等进行了分析。结果表明:8个GmDRT100基因位于5条大豆染色体上,具有相对保守的基因结构和保守基序,同时还具有多种与激素和非生物胁迫相关的顺式作用元件。RT-qPCR分析揭示8个GmDRT100家族成员在盐旱胁迫下均不同程度的上调表达,且GmDRT100-6在干旱和盐胁迫下均显著上调。进一步研究GmDRT100-6的功能,结果表明GmDRT100-6蛋白位于细胞膜中,大豆毛状根中GmDRT100-6基因的过表达提高了植株的耐旱性和耐盐性。这些研究结果表明GmDRT100-6在大豆抗旱、抗盐胁迫中发挥了重要作用。

DNA-damage repair/tolerance 100(DRT100)codes a type of protein rich in leucine repeats,which is widely involved in plant development and abiotic stress response processes.In order to identify DRT100 gene family members in soybean(Glycine max L.),reveal its evolutionary relationship and related functions of drought and salt tolerance in soybean.In this study,eight DRT100 genes were identified at the genome-wide level of soybean,and their structural characteristics,phylogeny,cis-acting elements of promoters,and gene expression patterns were analyzed.The results showed that eight GmDRT100 genes were located on five soybean chromosomes,and had relatively conserved gene structures and motifs,as well as multiple cis-acting elements related to hormone and abiotic stress.RT-qPCR analysis revealed that the 8 GmDRT100 genes were found to be up-regulated to varying degrees under salt and/or drought conditions,and GmDRT100-6 was significantly upregulated under drought and salt stress.Further studies on the function of GmDRT100-6 showed that Gm-DRT100-6 protein was located in the cell membrane,and the overexpression of GmDRT100-6 gene in hairy roots of soybean improved drought tolerance and salt tolerance of plants.These results indicated that GmDRT100-6 played an important role in drought and salt stress resistance of soybean.