基于巴戟天RNA-seq的bHLH转录因子的鉴定与分析

Identification and Analysis of the bHLH Transcription Factor Based on RNA-seq Database in Morinda officinalis

为探究巴戟天中参与生长发育及次生代谢产物合成的bHLH转录因子,本研究基于巴戟天的转录组数据,对巴戟天的bHLH转录因子进行筛选鉴定、亚细胞定位、保守结构域、GO功能注释、系统进化树及组织特异性表达差异分析。结果显示,鉴定出的90个巴戟天bHLH转录因子的保守结构域大约有60个氨基酸组成,包含2个不同的功能结构区,其中83个bHLH转录因子具有识别并结合E-box的能力。63个bHLH转录因子定位于细胞核中,27个位于细胞外基质。GO功能注释分类结果显示与生物过程、分子功能和细胞成分有关的GO terms数量分别为39、109和61个。系统进化树分析结果可知,除S2、S4、S12、S14、S17和S20亚家族外,90个巴戟天bHLH转录因子在其他亚家族均有分布。RT-qPCR结果验证了部分MobHLH转录因子在巴戟天不同组织中的表达模式。鉴定的90个巴戟天bHLH转录因子为后续进一步研究巴戟天bHLH转录因子家族提供了一定的理论基础。

To explore the bHLH transcription factors related to development and secondary metabolite biosynthesis in Morinda officinalis,this study was conducted to screen and identify the MobHLH transcription factors(TFs)based on the transcriptome data.The cellular localization,conserved structural domains,GO functional annotation,phylogenetic tree,and tissue-specific expression differences were analyzed.The results showed that the 90 identified MobHLH TFs had a conserved structural domain consisting of approximately 60 amino acids and contained two different functional structural regions,of which 83 MobHLH TFs had the ability to recognize and bind E-box.63 MobHLH TFs were localized in the nucleus and 27 were located in the extracellular.The results of GO functional annotation showed that 39,109,and 61 GO terms were classified into biological processes,molecular functions,and cellular components,respectively.Phylogenetic tree analysis showed that 90 MobHLH TFs were distributed in all subfamilies except subfamilies S2,S4,S12,S14,S17,and S20.RT-qPCR results verified the expression patterns of some MobHLH TFs in different tissues of M.officinalis.The identified 90 MobHLH TFs provided a theoretical basis for further study of the bHLH TFs in M.officinalis.