视网膜格子样变性玻璃体液中相关细胞因子表达

Expression profiles of cytokines in vitreous humor in lattice degeneration

目的观察孔源性视网膜脱离(RRD)伴格子样变性(LD)患眼玻璃体液中相关细胞因子表达水平。方法临床观察性研究。2022年5月至2023年2月于东南大学附属中大医院及南京医科大学附属眼科医院检查确诊且首次行玻璃体切割手术(PPV)治疗的RRD伴或不伴LD患者43例43只眼纳入研究。将患者分为RRD伴LD组(LD组)、RRD不伴LD组(Non-LD组),分别为27例27只眼、16例16只眼。选取同期特发性黄斑裂孔患者6例6只眼、特发性视网膜前膜患者4例4只眼作为对照组。PPV开始未开放眼内灌注前,切除并抽吸未稀释的中央部玻璃体液0.5 ml。采用Luminex高通量多因子检测技术定量检测玻璃体液中单核细胞趋化蛋白-1(MCP-1)、巨噬细胞炎症蛋白(MIP)-1α、MIP-1β、干扰素-γ诱导蛋白-10(IP-10)、白细胞介素(IL)-6、IL-8、巨噬细胞迁移抑制因子(MIF)、肿瘤坏死因子-α、干扰素-γ、细胞间黏附分子(ICAM)-1、血管细胞黏附分子(VCAM)-1、血小板内皮细胞黏附分子(PECAM)-1、胎盘生长因子(PLGF)、血管内皮生长因子(VEGF)浓度。组间细胞因子表达水平比较采用Kruskal-Wallis秩和检验,DSCF法校正事后两两比较的显著性水平。结果LD组、Non-LD组、对照组患眼IL-6(H=14.400)、IL-8(H=13.610)、MCP-1(H=12.050)、VEGF(H=9.920)、MIP-1α(H=6.620)、IP-10(H=7.780)、MIF(H=12.920)、PECAM-1(H=9.990)、ICAM-1(H=8.070)、PLGF(H=16.850)浓度比较,差异有统计学意义(P<0.05)。组间两两比较,LD组玻璃体液中IL-6、IL-8、PLGF浓度高于Non-LD组,差异有统计学意义(P<0.05)。结论RRD伴LD患眼玻璃体液中IL-6、IL-8、PLGF表达水平升高。

Objective To observe the expression levels of related cytokines in the vitreous humor of eyes with rhegmatogenous retinal detachment(RRD)associated with lattice degeneration(LD).Methods A clinical observational study.From May 2022 to February 2023,43 patients of 43 eyes diagnosed with RRD,with or without accompanying LD,who underwent their first pars plana vitrectomy(PPV)at Zhongda Hospital Southeast University and The Affiliated Eye Hospital of Nanjing Medical University were included in the study.The patients were divided into two groups:RRD with LD(LD group),consisting of 27 patients with 27 eyes,and RRD without LD(Non-LD group),consisting of 16 patients with 16 eyes.Additionally,6 patients(6 eyes)with idiopathic macular holes and 4 patients(4 eyes)with idiopathic epiretinal membranes during the same period were selected as the control group.Before initiating PPV and without intraocular perfusion,a 0.5 ml sample of undiluted vitreous fluid from the central portion was excised and aspirated.The concentrations of monocyte chemoattractant protein-1(MCP-1),macrophage inflammatory protein-1 alpha(MIP)-1α,MIP-1β,interferon-γ-inducible protein 10(IP-10),interleukin-6(IL-6),IL-8,macrophage migration inhibitory factor(MIF),tumor necrosis factor-alpha-α,interferon-γ,intercellular adhesion molecule 1(ICAM-1),vascular cell adhesion molecule-1,platelet endothelial cell adhesion molecule 1(PECAM-1),placental growth factor(PLGF)and vascular endothelial growth factor(VEGF)in the vitreous fluid were quantitatively measured using the Luminex high-throughput multiplex assay technology.The comparison of cytokine expression levels between groups was performed using the Kruskal-Wallis rank sum test,with significance levels for post-hoc pairwise comparisons adjusted by DSCF methods.Results The eyes of the patients in the LD group,Non-LD group,and control group showed statistically significant differences(P<0.05)in the concentrations of IL-6(H=14.400),IL-8(H=13.610),MCP-1(H=12.050),VEGF(H=9.920),MIP-1α(H=6.620),IP-10(H=7.780),MIF(H=12.920),PECAM-1(H=9.990),ICAM-1(H=8.070),and PLGF(H=16.850).Upon pairwise comparison between groups,the vitreous fluid concentrations of IL-6,IL-8,and PLGF in the LD group were found to be significantly higher than those in the Non-LD group(P<0.05).Conclusion The expression levels of IL-6,IL-8,and PLGF are elevated in the vitreous fluid of eyes with RRD accompanied by LD.