槲树不同外植体愈伤组织诱导及继代培养研究

Studies on callus tissue induction and subculture from different explants of Quercus dentata

【目的】以槲树(Quercus dentata)的根与叶为外植体,以期筛选出愈伤组织诱导和继代培养的适宜条件,为槲树组培快繁体系的建立提供支撑。【方法】在WPM、MS、1/2MS三种培养基中,加入不同浓度的NAA和6-BA,以槲树幼苗根与叶为外植体诱导愈伤组织,记录、分析和比较不同条件下的诱导率。再将根诱导后的愈伤组织接种于WPM培养基中,添加不同浓度的2,4-D和6-BA进行愈伤组织的增殖,计算和比较增殖系数。【结果】叶片诱导愈伤组织的最佳培养基为MS+NAA 1.0 mg/L+6-BA 0.5 mg/L。根诱导愈伤组织的最佳的培养基为WPM+NAA 2.0 mg/L+6-BA 1.0 mg/L。经比较,以根作为外植体的诱导率高于叶片。愈伤组织增殖的最佳培养基为WPM+2,4-D 1.2 mg/L+6-BA 0.9 mg/L。【结论】筛选出了最适合槲树愈伤组织诱导和增殖的培养基配方,为槲树组培快繁体系的建立奠定了基础。

[Objective]In this study,the roots and leaves of Quercus dentata were used as explants to screen the optimal conditions of callus tissue induction and subculture,which can support the establishment of tissue culture and rapid propagation system for Q.dentata.[Methods]In the WPM,MS and 1/2MS medium containing different concentrations of NAA and 6-BA,the explants of roots and leaves from.Q.dentata seedling were cultured to induce callus tissue,and the induction rates were recorded,analyzed,and compared.Then the induced callus tissue from roots was inoculated into WPM medium with different concentrations of 2,4-D and 6-BA to proliferate,and the proliferation coefficients were calculated and compared.[Results]The optimal medium for the induced callus tissue of leaves was MS medium containing 1.0 mg/L NAA and 0.5 mg/L6-BA.The optimal medium for the induced callus tissue of roots was WPM medium containing 2.0 mg/L NAA and 1.0 mg/L 6-BA.After comparison,the induction rate of root explants was higher than that of leaves.WPM medium containing 1.2 mg/L 2,4-D and 0.9 mg/L 6-BA was found to be optimal for callus tissue proliferation.[Conclusion]In this study,the optimal medium formulation for the induction and proliferation of Q.dentata callus tissue was successfully screened out,which laid the foundation for establishing tissue culture and rapid propagation system of Q.dentata.