血清HDAC4和HDAC6的表达与子痫前期发生及病情严重程度的相关性分析

Correlation between the expression of HDAC4 and HDAC6 in serum and the occurrence and severity of preeclampsia

目的 检测子痫前期患者血清组蛋白去乙酰化酶4(HDAC4)、组蛋白去乙酰化酶6(HDAC6)的表达水平,分析二者与病情严重程度的相关性。方法 选取2020年1月—2021年6月于西北妇女儿童医院住院治疗的80例子痫前期患者,根据患者病情的严重程度分为轻度组(n=48)和重度组(n=32),另选取同期产检并住院分娩的健康孕妇80例为对照组。比较子痫前期患者组和对照组一般资料;Pearson相关性分析血清HDAC4水平、HDAC6水平与子痫前期患者病情严重程度的相关性,以及血清HDAC4水平与血清HDAC6水平的相关性;多因素Logistic回归分析影响孕妇发生子痫前期的因素;受试者工作特征(ROC)曲线分析血清HDAC4、HDAC6水平对子痫前期发生的评估价值。结果 子痫前期患者收缩压、舒张压、24 h尿蛋白定量、总胆固醇、甘油三脂、HDAC4、HDAC6显著高于对照组(P<0.05);重度组HDAC4、HDAC6表达水平显著高于轻度组(P<0.05)。相关性分析显示,血清HDAC4、HDAC6与病情严重程度呈显著正相关(r=0.536、0.614,P均<0.001);子痫前期患者血清HDAC4水平与HDAC6水平呈显著正相关(r=0.412,P<0.001)。多因素Logistic回归分析显示,血清HDAC4、HDAC6、收缩压、舒张压、24 h尿蛋白定量、总胆固醇、甘油三脂是发生子痫前期的影响因素(P<0.05)。血清HDAC4、HDAC6二者联合预测子痫前期发生的AUC为0.886,敏感度为87.50%,特异性为78.75%,优于各自单独预测(Z_(联合检测-HDAC4)=2.564、Z_(联合检测-HDAC6)=4.083,P均<0.05)。结论 子痫前期患者血清HDAC4、HDAC6水平与疾病的发生和严重程度密切相关,二者联合对孕妇发生子痫前期有较好参考价值。

Objective To detect the expression levels of serum histone deacetylase 4(HDAC4)and histone deacetylase 6(HDAC6)in patients with preeclampsia,and analyze the correlation between the two and the severity of the disease.Methods Eighty preeclampsia patients(study group)hospitalized in Northwest Women and Children′s Hospital from January 2020 to June 2021 were selected,the patients were grouped into mild group(n=48)and severe group(n=32)according to the severity of their illness,in addition,80 healthy pregnant women who gave birth in hospital during the same period were regarded as the control group.The general data of the study group and the control group were compared;Pearson correlation analysis was applied to analyze the correlation between serum HDAC4 level,HDAC6 level and the severity of preeclampsia,and the correlation between serum HDAC4 level and serum HDAC6 level;multivariate Logistic regression analysis was applied to analyze the factors influencing the occurrence of preeclampsia in pregnant women;the evaluation value of serum HDAC4 and HDAC6 levels on the occurrence of preeclampsia was analyzed by receiver operating characteristic(ROC)curve.Results Systolic blood pressure,diastolic blood pressure,24h urine protein content,total cholesterol,triglyceride,HDAC4,and HDAC6 in preeclampsia patients were obviously higher than those in the control group(P<0.05);the expression levels of HDAC4 and HDAC6 in severe group were obviously higher than those in mild group(P<0.05).Correlation analysis showed that serum HDAC4 and HDAC6 were obviously positively correlated with the severity of the disease(r=0.536,0.614,P<0.001);there was a obvious positive correlation between serum HDAC4 level and HDAC6 level in patients with preeclampsia(r=0.412,P<0.001).Multivariate Logistic regression analysis showed that serum HDAC4,HDAC6,systolic blood pressure,diastolic blood pressure,24h urine protein content,total cholesterol,and triglyceride,were the influencing factors of preeclampsia(P<0.05).The AUC of combined prediction of serum HDAC4 and HDAC6 for the occurrence of preeclampsia was 0.886,the sensitivity was 87.50%,and the specificity was 78.75%,which was superior to their respective independent prediction(Z_(combined detection-HDAC4)=2.564,Z_(combined detection-HDAC6)=4.083,P<0.05).Conclusion The serum HDAC4 and HDAC6 levels in patients with preeclampsia are closely related to the occurrence and severity of the disease,and the combination of the two has a good reference value for pregnant women with preeclampsia.