8-羟基喹啉对小鼠肝脏氧化损伤及微核实验研究

Experimental Study on Oxidative Damage of Liver and Micronucleus Induced by 8-Hydroxyquinoline in Mice

研究8-羟基喹啉(8-HQ)对小鼠肝脏氧化损伤及微核实验。方法:设低中高剂量8-HQ组、溶剂对照组、阳性对照组、空白组共六个组,连续灌胃给药7 d后,检测血清中谷丙转氨酶(ALT)、谷草转氨酶活性(AST),解剖小鼠检测肝脏脏器系数、小鼠肝组织内脂质过氧化产物丙二醛(MDA)含量以及抗氧化指标过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活性。结果:与对照组比较,溶剂组雌雄小鼠肝脏脏器系数、ALT、AST活力、MDA含量及抗氧化指标无显著差异;8-羟基喹啉各剂量染毒组的小鼠明显比空白组小鼠的SOD酶、CAT酶、GSH-PX酶活性显著降低(p<0.05)、高浓度组的差异极显著(p<0.01);高剂量组的8-羟基喹啉致雌雄小鼠骨髓细胞微核形成率较显著(p<0.05))。结论:17.5~70 mg/kg 8-羟基喹啉对小鼠的肝脏具有氧化损伤作用,70 mg/kg 8-羟基喹啉对小鼠具有致突变影响。

To inestigate the liver oxidative damage and micronucleus formation rate of micronucleus in mice,which treated by different concentrations of 8-hydroxyquinoline.Three dosse groups,positive control(cyclophosphamide),solvent control and blank control were set.After continuous intragastric administration for 7 days,serum alanine aminotransferase(ALT)and aminotransferase(AST)activities were detected.The liver organ coefficient,the content of lipid peroxidation product malondialdehyde(MDA)and the activities of antioxidant indexes catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in liver tissue of mice were detected.Results:Compared with the control group,there were no significant differences in liver organ coefficient,ALT,AST activity,MDA content and antioxidant index of male and female mice in solvent group.The activities of SOD enzyme,CAT enzyme and GSH-PX enzyme in 8-hydroxyquinoline group were significantly decreased compared with those in blank group(p<0.05),and the differences in high concentration group were extremely significant(p<0.01).The micronucleus formation rate of male and female mice induced by 8-hydroxyquinoline was significantly higher in high dose group(p<0.05).Conclusion:17.5~70 mg/kg 8-hydroxyquinoline has liver oxidative damage and mutagenic effect of mice.