叶酸代谢酶MTHFR基因多态性与复发性流产相关性研究

Association study between folate metabolizing enzyme MTHFR gene polymorphism and recurrent abortion

目的探讨复发性流产(RSA)与叶酸代谢酶MTHFR基因C677T和A1298C、MTRR基因A66G多态性的相关性。方法选取2022年08月-2023年11月在新疆巴音郭楞蒙古自治州人民医院不孕不育科及妇产科就诊并确诊为复发性流产的非妊娠患者121例为RSA组,另选取同时期在本院进行健康保健咨询的育龄期女性129例为正常对照组。采用PCR金磁微粒法分析两组人群MTHFR C677T和A1298C、MTRR基因A66G位点的基因多态性。结果两组基因型比较,复发性流产组MTHFR基因C677T位点TT型和A1298C位点CC型、MTRR基因A66G位点GG型的分布频率均高于健康组(P<0.05)。复发性流产组叶酸高风险比率(8.26%)比健康组叶酸高风险比率(1.55%)高,且低风险的比率(22.31%)比健康组(37.21%)低(P<0.05)。结论MTHFR C677T的T等位基因和A1298C的C等位基因、MTRR基因A66G的G等位基因可能增加巴州地区人群复发性流产发生风险。

Objective To investigate the correlation between recurrent miscarriage(RSA)and polymorphisms of the folate metabolising enzyme MTHFR genes C677T and A1298C,MTRR gene A66G.Methods A total of 121 non-pregnant patients who attended the infertility department,gynaecology and obstetrics department of the People's Hospital of Bayingolin Mongol Autonomous Prefecture,Xinjiang,and were diagnosed with recurrent miscarriage from August 2022 to November 2023 were selected as the RSA group,and an additional 129 women of childbearing age who were undergoing health care consultation in our hospital during the same period were selected as the normal control group.Gene polymorphisms at the MTHFR C677T and A1298C,MTRR gene A66G loci were analyzed in the two groups by PCR gold magnetic particle method.Results Compared the genotypes of the two groups,the frequency of distribution of MTHFR gene TT type at C677T locus and CC type at A1298C locus,and GG type at MTRR gene A66G locus were higher in the recurrent miscarriage group than in the healthy group(P<0.05).The rate of high risk of folic acid in the recurrent miscarriage group(8.26%)was higher than the rate of high risk of folic acid in the healthy group(1.55%),and the rate of low risk(22.31%)was lower than that in the healthy group(37.21%)(P<0.05).Conclusion The t allele of MTHFR C677T and the C allele of A1298C,and the G allele of MTRR gene A66G may increase the risk of occurrence of recurrent miscarriage in the population of Baja region.