马铃薯气生茎腐病病原鉴定及其生物学特性研究

Pathogen identification and biological characteristics of potato aerial stem rot

马铃薯气生茎腐病是近年内蒙古马铃薯生产中新发生的一种细菌病害,在发病区域采集典型病样,分离病原菌,对其进行致病性测定、形态学和分子学鉴定,明确引起马铃薯气生茎腐病的病原菌为胡萝卜软腐果胶杆菌巴西亚种(Pectobacterium carotovorum subsp.brasiliense)。同时,对该病菌的部分生物学特性开展了室内研究,发现其最适生长pH为6.5,最适生长温度为28℃,最适碳源、氮源分别为甘露醇、硫酸铵,为后续开展该菌生物学及防治的研究提供了参考。

In recent years,aerial stem rot of potato has emerged as a new bacterial disease in the main potato-producing areas in Inner Mongolia.Samples with typical symptoms were collected in the diseased area and the pathogens were isolated.The pathogenicity test,morphological study,and molecular identification were carried out.The results showed that Pectobacterium carotovorum subsp.brasilensis was the pathogen responsible for causing potato aerial stem rot.The optimal growth conditions for the pathogen were found to be a pH of 6.5,a temperature of 28℃,and the carbon and nitrogen sources were mannitol and ammonium sulfate,respectively.These findings will serve as a practical reference for the subsequent research on the biology and control of the disease.