基于转录组测序的远红光辐照下水稻幼苗差异表达基因分析

Analysis of differentially expressed genes in rice seedlings under far-red light irradiation based on transcriptome sequencing

为探究远红光间断辐照下,水稻幼苗生长表型差异及其在基因层面的响应,实验分别设计远红光间断辐照5、10、15 min实验组与无远红光对照组,研究水稻幼苗生长差异,进行转录组基因测序,对差异表达基因进行注释分析。结果表明,经远红光间断辐照10 min,水稻种子发芽率增长30.2%。间断辐照10min组生长表型增长最为显著,根长、茎长、茎粗分别比对照组增加了35.64%、22.9%、18.92%。转录组测序共获得868个显著差异表达基因,其中筛选出44个显著上调基因,功能注释富集于植物激素信号传导与代谢、碳水化合物运输与代谢通路;119个显著下调基因,功能注释富集于次生代谢物、二萜类与苯丙烷类化合物的生物合成代谢通路。关键差异表达基因分析显示,远红光间断辐照显著增强植物激素代谢通路基因表达,主要增强了赤霉素、油菜素内酯和生长素的合成,使植物细胞伸长,显著促进水稻根茎生长。

In order to explore the differences of rice seedling growth phenotypes and their responses at the gene under intermittent far-red light irradiation,we designed the experiments of 5,10 and 15 min intermittent far-red light irradiation group and a control group without far-red light.After 18 days of incubation,the samples were taken for transcriptome gene sequencing.The analysis of differentially expressed genes took no far-red light as the control group(CK),and intermittent irradiation group with the most significant difference in growth phenotype as the experimental group(T).The results showed that the germination rate of rice seeds could be increased by up to 30%after intermittent irradiation with far-red light.868 differentially expressed genes were obtained by transcriptome sequencing.Among them,44 significantly up-regulated genes were screened,and functional annotation was enriched in plant hormone signal transduction and metabolism,carbohydrate transport and metabolism pathways.119 genes were significantly down regulated,and their functional annotation was enriched in the biosynthetic metabolic pathway of secondary metabolites,diterpenoids and phenylpropanes.Analysis of key differentially expressed genes showed that intermittent far-red light irradiation significantly enhanced gene expression of plant hormone metabolism pathway.The synthesis of gibberellin,brassinolide and auxin increased.Therefore,plant cells elongate and rice rhizome growth is significantly promoted.Analysis of key differentially expressed genes showed that intermittent far-red light irradiation significantly enhanced gene expression of plant hormone metabolism pathway.The synthesis of gibberellin,brassinolide and auxin increased.Therefore,plant cells elongate and rice rhizome growth is significantly promoted.