富硒油菜粉抗氧化特性及其与化学成分相关性

Antioxidant Properties and Correlation with Chemical Components in Selenium-Enriched Rape Powder

本研究分析富硒油菜粉提取物的体外抗氧化活性以及与基本化学成分之间的相关性。结果表明,相比于普通油菜粉提取物,富硒油菜粉提取物中含有更多含氮化合物、多酚、黄酮、总硒以及多糖硒,具有更高的还原Fe3+以及清除1,1-二苯基-2-三硝基苯肼自由基、2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基和羟自由基能力。相关性分析显示抗氧化活性与含氮化合物、总糖、多酚、黄酮、皂苷、多糖、总硒以及多糖硒含量均呈一定的正相关。偏相关性分析显示抗氧化活性仅与多糖和多糖硒含量呈显著或极显著正相关,这表明两种提取物抗氧化活性的差异可能主要由多糖和多糖硒含量差异造成。总之,富硒油菜粉提取物和普通油菜粉提取物的基本成分具有一定差异,且富硒油菜粉提取物具有更好的体外抗氧化活性,多糖硒是其主要的抗氧化成分。

This work aims to study the in vitro antioxidant activity of selenium-enriched rape powder extract and its correlation with basic chemical components.The results showed that compared with regular rape powder extract,selenium-enriched rape powder extract contained more nitrogen compounds,polyphenols,flavonoids,total selenium and polysaccharide-bound selenium,and had higher Fe3+reducing power,1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical cation,and hydroxyl free radical scavenging activity.Correlation analysis showed that the antioxidant activity was positively correlated with nitrogen compounds,total sugar,polyphenols,flavonoids,saponins,polysaccharides,total selenium,and polysaccharide-bound selenium.Partial correlation analysis showed that the antioxidant activity was only significantly or highly significantly positively correlated with polysaccharides and polysaccharide-bound selenium,which indicated that the difference in antioxidant activity between the two rape powder extracts may be mainly caused by the differences in polysaccharides and polysaccharide-bound selenium contents.In conclusion,there are differences in the basic chemical compositions of selenium-enriched and regular rape powder extracts,and selenium-enriched rape powder extract has a higher in vitro antioxidant activity.Polysaccharide-bound selenium was the major antioxidant component.