摘要
柳树(Willow)是杨柳科(Salicaceae)柳属(Salix)乔木植物,大多数品种具有耐旱耐盐碱的特性,对环境具有良好的适应能力[1]。柳树在甘肃省内广泛种植,是风景林、防护林和水土保持林的重要组成树种。柳树丛枝病是一种典型的植原体病害,当寄主植株被病原物侵染时,顶芽生长受到抑制而侧芽提前萌发,导致受害枝条无法正常抽条或木质化,严重时甚至引起整株枯死[2],对林业的生态、经济和社会效益造成了极大损失。近年来,柳树丛枝病在甘肃省各市州发生严重,已经引起省内各界的普遍关注。
To clarify the taxonomic status of the phytoplasma causing willow witches’-broom in Gansu Pro-vince,this study used nested PCR technique to clone the 16S rDNA gene using total DNA collected from differ-ent regions and species of willow witches’-broom samples as templates,and nine 1250 bp target fragments were obtained using P1/P7 and R16F2n/R16R2;the sequences of the above genes were compared by homolo-gy,The results showed that the 16S rDNA gene of willow witches’-broom phytoplasma strain had the highest homology with Willow proliferation phytoplasma(Willow proliferation)in Inner Mongolia,both above 99.1%;the phylogenetic tree was constructed and found that the 16S rDNA gene of willow witches’-broom clustered with members of 16SrVI-A subgroup.The phylogenetic tree was constructed,and the 16S rDNA gene of willow witches’-broom clustered with members of the 16SrVI-A subgroup;and the virtual RFLP analysis reported that the highest sequence similarity with the one obtained in this study was the reference pattern of the 16SrVI-A subgroup,with a similarity coefficient around 0.98,so the willow witches’-broom phytoplasma in Gansu Province could be classified into the 16SrVI-A subgroup.Meanwhile,the phytoplasma resources were preserved by grafting and identified as vector insects based on amplification of the 16S rDNA gene in the willow shoulder reticulata.
作者
陈雅寒
武崇高
方健惠
朱亚灵
许宏刚
程晓月
欧阳经政
CHEN Yahan;WU Chonggao;FANG Jianhui;ZHU Yaling;XU Honggang;CHENG Xiaoyue;OUYANG Jingzheng(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China;Forestry pest control and quarantine station of Gansu,Lanzhou 730070,China;Institute of Landscape Science,Lanzhou 730070,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2024年第1期220-224,共5页
Acta Phytopathologica Sinica
基金
甘肃农业大学人才启动金项目(GAU-KYQD-2019-22)
林业科技创新与国际合作省级财政资金项目([2019]418)
兰州市科技局项目(2022-2-49)
国家自然科学基金(32160628)
甘肃省自然科学基金(21JR7RA820)
甘肃省高等学校创新基金项目(2021B-117)。