密斑刺鲀鱼皮胶原蛋白对UVB诱导小鼠皮肤急性光损伤的保护作用

Protective Effect of Collagen from Diodon Hystrix Skin on UVB⁃induced Acute Skin Photodamage in Mice

初步探究密斑刺鲀鱼皮胶原蛋白(Diodon hystrix skin collagen,DHSC)对紫外线B(ultraviolet B,UVB)诱导的小鼠皮肤急性光损伤的保护作用。将Balb/c小鼠分为正常组(CTL)、模型组(MOD)、DHSC低剂量组(L)、DHSC中剂量组(M)、DHSC高剂量组(H)、基质对照组(B)、阳性对照组(Y),以UVB诱导法建立小鼠皮肤急性光损伤模型。实验结束后观察DHSC对小鼠背部皮肤的作用情况并进行双盲法红斑评分,HE染色后观察DHSC对皮肤组织病理的影响,Masson染色后观察组织中胶原蛋白的结构,并通过比色法检测组织中超氧化物歧化酶(superoxide dismutase,SOD)的活力及丙二醛(malondialdehyde,MDA)的含量。结果表明,密斑刺鲀鱼皮胶原蛋白可以有效减轻UVB辐射引起的小鼠皮肤外观形态、组织结构及胶原蛋白组织的改变,通过抑制抗氧化酶活性降低和减少脂质过氧化物堆积,表现出对小鼠皮肤急性光损伤的保护作用。

To explore the protective effect of Diodon hystrix skin collagen(DHSC)on UVB⁃induced acute skin photo⁃damage in mice,Balb/c mice were divided into control group(CTL),model group(MOD),low dose DHSC group(L),me⁃dium dose DHSC group(M),high dose DHSC group(H),matrix control group(B),and positive control group(Y).Skin acute photodamage model was established by UVB induction method.At the end of the experiment,the effect of DHSC on the back skin of mice was observed and scored by the double blind erythema.The effect of DHSC on skin histopathology was observed by HE staining.The structure of collagen in the tissue was observed by Masson staining.The activity of super⁃oxide dismutase(SOD)and the content of malondialdehyde(MDA)in the tissue were detected by colorimetry.The results showed that DHSC could effectively alleviate the UVB radiation⁃induced changes of skin appearance,tissue structure and collagen tissue in mice,and exhibited the protective effect on acute light injury of mice’s skin by inhibiting the reduction of antioxidant enzyme activity and reducing the accumulation of lipid peroxide.