藜麦热激蛋白60(CqHSP60)基因家族生物信息学和表达模式分析

Bioinformatics and Expression Pattern Analysis of Heat Shock Proteins 60 Gene(CqHSP60)Family of Quinoa(Chenopodium quinoa Willd.)

为了探究藜麦HSP60基因(CqHSP60)的结构特征、基因功能和表达模式,基于藜麦全基因组和转录组数据,利用生物信息学方法对CqHSP60基因家族进行鉴定和系统分析。依据亚细胞结构定位分为了三个亚组,每个亚组具有相似的Motif和基因结构;通过RNA-Seq数据对CqHSP60基因在不同发育阶段进行了表达分析,并对在热、干旱、盐胁迫等不同处理条件下CqHSP60基因的胁迫响应进行了分析,结果表明CqHSP60基因在花簇和干种子中表达量较高,并对不同的胁迫模式均有响应;在CqHSP60基因的启动子区发现了多个与胁迫相关的顺式调控元件,这表明这些基因在多重胁迫下受到应激响应。

In order to explore the structural characteristics,gene function and expression pattern of quinoaHSP60 gene(CqHSP60),the CqHSP60 gene family is identified and systematically analyzed by using bioinformatics methods.The result showes that 37 CqHSP60 genes are identified in the quinoa genome,which can be divided into 3 phylogenetic subgroups according to subcellular structure localization,and each subgroup has similar motif and gene structure.Through RNA-Seq data,the gene expression of tissues at different developmental stages and different treatments such as heat,drought and salt stress are analyzed.CqHSP60 genes respond to different stress,and express higher in flower clusters and dried seeds.Multiple stress-associated cis-regulatory elements are identified in the promoter region of the CqHSP60 gene,suggesting that these genes are subjected to stress responses under multiple stresses.