转基因107杨抗虫性研究

Study on insect resistance of transgenic Populus 107

为研究转基因107杨对靶标昆虫柳蓝叶甲的抗虫性以及对非靶标昆虫白杨毛蚜及天敌昆虫异色瓢虫生长发育的影响,以室内饲养的方法,比较了取食转基因107杨及对照的柳蓝叶甲各龄期幼虫的死亡率、研究了取食转基因107杨及对照107杨叶片的白杨毛蚜生长发育情况,以及取食不同株系107杨上白杨毛蚜和柳蓝叶甲的异色瓢虫幼虫的生长发育情况。结果表明,转基因107杨对靶标昆虫柳蓝叶甲幼虫表达高抗虫性;取食转基因107杨叶片的白杨毛蚜幼虫期发育时间以及生命参数与对照相比均无显著差异;取食转基因107杨上白杨毛蚜的异色瓢虫幼虫发育历期及体长体宽与对照相比无显著差异;取食转基因107杨上柳蓝叶甲的异色瓢虫幼虫发育历期及体长体宽与对照相比无显著差异。研究证明,转基因107杨对非靶标昆虫白杨毛蚜和天敌昆虫异色瓢虫生长发育无显著影响,转基因107杨生态安全性较高。

In order to study the insect resistance of transgenic Populus 107 to the target insect,Plagiodera versicolora,as well as the effects on the growth and development of non-target insect,Chaitophorus populeti,and natural enemy insect,Harmonia axyridis.Providing theoretical basis for the ecological safety of transgenic Populus 107,we compared the mortality rate of P.versicolora larvae at each age feeding on transgenic Populus 107 and control,and studied the growth and development of C.populeti between the transgenic Populus 107 group and control group.Meanwhile,we analyzed the growth and development of H.axyridis larvae feeding on P.versicolora and C.populeti on transgenic Populus 107 and control by laboratory experiments.The results showed that the transgenic Populus 107 expressed high insect resistance to the target insect,P.versicolora larvae.There were no significant differences in the development time and life parameter of C.populeti larvae feeding on the leaves of the transgenic Populus 107 compared with the control.There were no significant differences in the development time as well as the body length and width of H.axyridis larvae preying on either C.populeti or P.versicolora fed on the transgenic Populus 107 compared with the control.In conclusion,this study demonstrated that the transgenic Populus 107 had no significant effect on the growth and development of the non-target insect C.populeti and the natural enemy insect H.axyridis,and the ecological safety of the transgenic Populus 107 was high.