卵巢癌组织中PTDSS1表达及临床意义

Expression of PTDSS1 in ovarian cancer and its clinical significance

目的探讨卵巢癌组织中PTDSS1的基因和蛋白表达及其临床意义。方法使用GEPIA网站在线数据库比较卵巢癌组织和正常组织PTDSS1表达水平,并分析PTDSS1表达与患者卵巢癌预后的关系。收集2015年至2020年间在衡水市第二人民医院妇科经手术治疗的94例卵巢癌患者作为研究对象,采用免疫组化染色法检测石蜡包埋组织PTDSS1蛋白表达。比较PTDSS1蛋白表达卵巢癌临床病理特征的关系。Kaplan-meier法绘制生存曲线,生存差异行Log-rank检验。采用Cox风险比例回归模型分析影响卵巢癌预后的因素。结果GEPIA网站在线数据库分析结果显示,卵巢癌中PTDSS1相对表达水平高于正常组织(P<0.05)。免疫组化染色分析显示,卵巢癌组织中PTDSS1蛋白水平显著高于癌旁组织(P<0.05)。PTDSS1蛋白表达与年龄、分化程度无关(P>0.05);与TNM分期、T分期和N分期有关(P<0.05)。根据PTDSS1免疫组化评分,发现I期卵巢癌中PTDSS1表达最低,与I期比较,Ⅱ~Ⅳ期患者表达均显著增加(P<0.01)。GEPIA在线分析显示PTDSS1基因低表达患者生存时间优于PTDSS1基因高表达患者(P<0.05)。Kaplan-Meier法显示PTDSS1高表达患者中位生存时间为18个月,显著低于PTDSS1低表达患者的36个月(P<0.01)。单因素和多因素Cox风险比例回归模型显示,TNM分期和PTDSS1蛋白表达水平影响术后卵巢癌患者OS的影响因素(P<0.05)。结论PTDSS1在卵巢癌组织中高表达,且预示预后不良。

Objective To investigate the expression of PTDSS1 gene and protein in ovarian cancer and its clinical significance.Methods The expression level of PTDSS1 in ovarian cancer tissues and normal tissues was compared using GEPIA online database,and the relationship between PTDSS1 expression and prognosis of patients with ovarian cancer was analyzed.The expression of PTDSS1 protein in paraffin-embedded tissues was detected by immunohistochemical method in 94 patients with ovarian cancer who underwent surgical treatment in gynecology department of our hospital from 2015 to 2020.To compare the relationship between PTDSS1 protein expression and clinicopathological features of ovarian cancer.Survival curve was drawn by Kaplan-meier method,and survival difference was tested by Log-rank.Cox proportional regression model was used to analyze the prognostic factors of ovarian cancer.Results The GEPIA online database analysis showed that the relative expression level of PTDSS1 in ovarian cancer was higher than that in normal tissues(P<0.05).Immunohistochemical staining analysis showed that PTDSS1 protein level in ovarian cancer tissues was significantly higher than that in adjacent tissues(P<0.05).The expression of PTDSS1 was not correlated with age or differentiation degree(P>0.05).It was related to TNM stage,T stage and N stage(P<0.05).According to the immunohistochemical score of PTDSS1,it was found that the expression of PTDSS1 in stage I ovarian cancer was the lowest,and the expressions of Ⅱ to Ⅳ were significantly increased compared with stage I ovarian cancer(P<0.01).GEPIA online analysis showed that the survival time of patients with low expression of PTDSS1 gene was better than that of patients with high expression of PTDSS1 gene(P<0.05).Kaplan-Meier method showed that the median survival time of patients with high PTDSS1 expression was 18 months,which was significantly lower than 36 months of patients with low PTDSS1 expression(P<0.01).Univariate and multivariate Cox proportional regression models showed that TNM stage and PTDSS1 protein expression level affected the influencing factors of OS in postoperative ovarian cancer patients(P<0.05).Conclusion PTDSS1 is highly expressed in ovarian cancer tissues and predicts poor prognosis.