CUMS诱导的抑郁症对小鼠海马神经元及炎症相关通路的影响

Effects of CUMS-induced Depression on Hippocampal Neurons and Inflammatory Pathways in Mice

为探讨慢性不可预知应激(CUMS)诱导的抑郁症对小鼠海马神经元及炎症相关通路的影响。试验选取12只8周龄C57BL/6雄性小鼠,平均分为2组(n=6):对照组、CUMS组,建立慢性不可预知应激模型。试验对2组小鼠进行糖水偏好试验、强迫游泳试验;采用尼氏染色观察小鼠脑组织海马区神经元变化;采用Western blot、qRT-PCR对小鼠海马区相关炎症因子(TNF-α、IL-1β、iNOS、IL-6)表达量进行检测。与对照组相比,CUMS组小鼠糖水偏好指数降低,但在水中静止时间增加;CUMS组小鼠海马区CA1、CA3及DG区尼氏染色阳性细胞数量显著下降,海马区炎症因子(TNF-α、IL-1β、iNOS、IL-6)蛋白表达量及m RNA表达量显著增加。CUMS诱导的抑郁症会导致小鼠海马神经元数量减少,并促进神经炎症的发生。

To explore the effects of chronic unpredictability stress(CUMS)induced depression on hippocampal neurons and inflammatory pathways in mice,twelve C57BL/6 male mice were selected and divided into two groups(n=6):control group and CUMS group,and chronic unpredictable stress model was established.Two groups of mice were tested for sugar water preference and forced swimming.Nissner staining was used to observe the changes of neurons in the hippocampus of mouse brain.Western blot and qRT-PCR were used to detect the expression levels of inflammatory factors(TNF-α、IL-1β、iNOS and IL-6)in the hippocampus of mice.Compared with the control group,the sugar water preference index of mice in CUMS group decreased,but the rest time in water increased.The number of Nis-stained positive cells in CA1,CA3 and DG in the hippocampus of CUMS group was significantly decreased,and the protein and mRNA expressions of inflammatory factors(TNF-α,IL-1β,iNOS and IL-17)in the hippocampus were significantly increased.CUMS-induced depression could reduce the number of hippocampal neurons and promote neuroinfla-mmation in mice.