藏香猪流行性腹泻病毒的PCR检测及毒株分型

Diagnostic and Gene Detection of Epidemic Diarrhea Virus Infection in Tibetan Scent Pigs

为明确四川省凉山彝族自治州某藏香猪规模化养殖场哺乳仔猪腹泻的病因,试验采集腹泻仔猪的粪便和肠道样品进行实验室病原学诊断,采用RT-PCR方法检测4种常见肠道病毒的感染情况。检测结果显示,猪流行性腹泻病病毒(PEDV)检测为阳性,猪delta冠状病毒、猪传染性胃肠炎病毒和猪A群轮状病毒3种病毒均为阴性。进一步采用RT-PCR扩增该毒株S1基因序列并测序。结果显示,该毒株与G2型变异毒株同源性最高,与其他基因型毒株同源性相对较低,因此判定该猪场仔猪发病是由猪流行性腹泻病毒G2型变异毒株感染所致。对病猪进行紧急免疫接种、治疗、消毒等综合防控措施,控制住了疫病的蔓延和发展。总结此次猪流行性腹泻病的治疗经验,为养猪场对该疫病的治疗和防控提供借鉴。

To clarify the causes of piglet diarrhea in large-scale Tibetan scent pig farm in Liangshan Yi Autonomous Prefecture,Sichuan Province.Fecal and intestinal samples of diarrhoeic piglets were collected for laboratory aetiology diagnosis.RT-PCR was used to detect the infections of four common intestinal viruses.The test results showed that porcine epidemic diarrhea virus(PEDV)antibody was positive.Porcine delta coronavirus,porcine infectious gastroenteritis virus and porcine group A rotavirus antibody were negative for all three viruses.The S1 gene sequence of this strain was further amplified by RT-PCR and sequenced.The results showed that the strain had the highest homology with the G2 variant strain and relatively low homology with other genotypes,so it was determined that the disease in piglets in this farm was caused by infection with the G2 variant strain of porcine epidemic diarrhea virus.Emergency immunization,treatment,disinfection and other comprehensive prevention and control measures were carried out on the sick pigs to control the spread of the disease.Summarized the treatment experience of this swine epidemic diarrhea virus epidemic,for pig farms to provide reference for the treatment,prevention and control of this epidemic.