纤维素酶辅助提取马齿苋总黄酮工艺优化及抑菌分析研究

Optimization of cellulase-assisted extraction of total flavonoids from Portulaca oleracea and its bacteriostatic analysis

用纤维素酶处理马齿苋,利用响应面设计,从纤维素酶添加量、酶解时间、酶解温度、酶解pH值四个因素优化纤维素酶处理后的黄酮提取率。对马齿苋提取物粗粉中的总黄酮进行抑菌试验。结果表明:脱脂后的马齿苋经测定黄酮类化合物含量为5.32%。最佳工艺条件为酶解温度50℃,酶的添加量0.6%,酶解时间1.8 h,酶解的pH值4.5,所得黄酮提取率6.09%,与未经酶处理相比,提取率提高1.14倍。对验证试验的黄酮粗粉进行抑菌试验,表明对金黄色葡萄球菌和大肠杆菌均有抑制作用,其中对大肠杆菌的抑菌效果较好。马齿苋经过纤维素酶酶解后,黄酮分子从细胞内溶出,提高马齿苋黄酮的提取率,提取的黄酮粗粉有抑菌活性,本研究为马齿苋黄酮的分离纯化、功能性研究提供支持。

Portulaca oleracea was treated with cellulase,and the extraction rate of total flavonoids from Portulaca oleracea was optimized using response surface method,cellulase addition amount,enzymatic hydrolysis time,enzymatic hydrolysis temperature,and enzymatic hydrolysis pH value were selected as influencing factors.Antibacterial tests of the total flavonoids in the crude extract of Portulaca oleracea was carried out.The results showed that the content of flavonoids in defatted Portulaca oleracea was 5.32%.The optimal extraction conditions were as follows:enzymatic hydrolysis temperature of 50℃,enzyme addition of 0.6%,enzymatic hydrolysis time of 1.8 hours,pH value of enzymatic hydrolysis of 4.5,and the extraction rate of total flavonoids from Portulaca oleracea was 6.09%,which was 1.14 times higher than that in Portulaca oleracea without enzymatic treatment.The antibacterial test showed that the total flavonoids in the crude extract of Portulaca oleracea had inhibitory effect on Staphylococcus aureus and Escherichia coli,and its antibacterial effect on Escherichia coli was better.With cellulase hydrolysis,flavonoid molecules were dissolved from the cells of Portulaca oleracea,improving the extraction rate of total flavonoids from Portulaca oleracea.The total flavonoids in the crude Portulaca oleracea extract had antibacterial activity,this paper provides support for the separation,purification,and functional research of flavonoids from Portulaca oleracea.