积雪草酸通过调控TLR4/NF-κB/NLRP3信号通路改善非酒精性脂肪肝的机制研究

Mechanism of asiatic acid improving nonalcoholic fatty liver disease by regulating TLR4/NF-κB/NLRP3 signaling pathway

探究天然活性成分积雪草酸(asiatic acid,AA)对高脂饮食导致的非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)的改善作用及机制。取雄性C57BL/6J小鼠40只,分为正常组、模型组、AA低剂量组、AA高剂量组,高脂饮食4周后给予AA干预,持续12周;实验结束后,称量肝脏重量并计算肝脏指数;通过分光光度法测定小鼠血清中脂肪肝相关生化指标的含量;通过酶联免疫吸附(enzyme linked immunosorbent assay,ELISA)法检测小鼠血清中相关炎症因子的表达水平;通过免疫印记法检测小鼠肝脏中TLR4/NF-κB/NLRP3信号通路相关蛋白的表达;通过多种染色法观察小鼠肝脏病理学变化。结果显示:AA可以有效改善NAFLD小鼠的一般指标,生化指标、炎症因子及TLR4/NF-κB/NLRP3信号通路相关蛋白的表达。AA可以通过下调TLR4/NF-κB/NLRP3信号通路,抑制肝脏炎症,改善NAFLD。

To investigate the effect and mechanism of the natural active ingredient asiatic acid(AA)on nonalcoholic fatty liver disease(NAFLD)induced by high-fat diet.Forty male C57BL/6J mice were divided into normal group,model group,AA low dose group and AA high dose group.After 4 weeks of high-fat diet,AA intervention was given for 12 weeks.After the experiment,the weight of mice livers and the mice liver index were measured.The content of biochemical indicators related to mice fatty liver in mice serum were measured by Spectrophotometer.The expression level of related inflammatory factors in mice serum were detected by enzyme linked immunosorbent assay.Proteins related to TLR4/NF-κB/NLRP3 signaling pathway in mice liver were detected by Western blotting.The pathological changes of liver in mice were observed by various staining methods.The results showed that AA could effectively improve the NAFLD mice general indicators,biochemical indicators,inflammatory factors,and proteins related to TLR4/NF-κB/NLRP3 signaling pathway.AA could inhibit liver inflammation and ameliorate NAFLD by downregulating TLR4/NF-κB/NLRP3 signaling pathway.