儿茶酚基团改性冷水鱼皮明胶组织粘合剂的制备及性能评价

Preparation and evaluation of catechol modified coldwater fish skin gelatin tissue adhesive

以冷水鱼皮明胶(FSGltn)为主体材料,通过定量修饰制备儿茶酚基团(CAT)改性明胶(CAT-FSGltn),再将CAT-FSGltn与葡聚糖二醛(DDA)原位混合交联,构建在湿润环境下具有高粘附性的组织粘合剂(CAT-FSGltn/DDA)。发现CAT-FSGltn/DDA能够在60 s内成胶。充分交联的CAT-FSGltn/DDA水凝胶在PBS溶液中浸泡超过80 h后无明显质量损失。将CAT-FSGltn/DDA粘附在模拟组织膜的橡胶薄膜上,当薄膜被拉伸至原长度的两倍时,水凝胶仍未断裂。利用体外模型评价组织粘合剂的耐压强度,结果显示CAT-FSGltn/DDA的耐压强度可达到105.7 mmHg,大于人体舒张压,表明CAT-FSGltn/DDA具有较强的内聚力和粘附力。组织切片显示固化30 min的CAT-FSGltn/DDA与肠衣表面贴合紧密,水凝胶/肠衣的界面在水流冲击后保持完好。细胞实验结果显示L929细胞的存活率在80%以上,CAT-FSGltn/DDA水凝胶的生物相容性良好。

Cold water fish skin gelatin(FSGltn)was chosen as the main material,wherein catechol groups(CAT)were linked through quantitative modification to prepare a catechol-modified gelatin(CAT-FSGltn).Then CAT-FSGltn and dextran dialdehyde(DDA)were mixed in situ to prepare a tissue adhesive(CAT-FSGltn/DDA)with good adhesion in a wet environment.It was found that the gelation time of CAT-FSGltn/DDA was within 60 s.The fully crosslinked CAT-FSGltn/DDA hydrogel showed minimal mass loss after being immersed in PBS solution for over 80 h.When adhered to a rubber film resembling the tissue membrane,the hydrogel CAT-FSGltn/DDA demonstrated an ability to withstand stretching up to twice its initial length without fracturing.An in vitro model was used to evaluate the compressive strength of the tissue adhesive.The compressive strength of CAT-FSGltn/DDA was observed to reach 105.7 mmHg,surpassing the human diastolic pressure.The results indicated that CAT-FSGltn/DDA possessed both strong cohesion and adhesion.Tissue sections showed that CAT-FSGltn/DDA cured for 30 min was closely attached to the casing surface,and the hydrogel/casing interface remained intact after the water flow impact.Cell experiments showed that the survival rate of L929 cells was more than 80%,CAT-FSGltn/DDA hydrogel had good biocompatibility.