延边州晒烟放线菌分离鉴定与系统发育多样性分析

Isolation and Identification of Actinomycetes from Sun-cured Tobacco in Yanbian Prefecture and Its Phylogenetic Diversity Analysis

为了获取不同环境和生态位对晒烟可培养放线菌的影响,从延边州龙井、图们和延吉3个不同地点的晒烟生长区取样,采用纯培养的方法,从烟草非根际土、根际土、根、茎、叶中分离晒烟放线菌。根据菌体形态和16S rDNA部分序列测定及GenBank-BLAST同源性分析进行菌种鉴定,共得到链霉菌属和红球菌属放线菌11株,且延吉晒烟样品分离出的菌株数量多于龙井和图们。对烟草不同部位的放线菌进行对比分析发现,分离到的放线菌数量由多到少依次为根、茎、叶。该研究结果有利于进一步解决晒烟种植过程中的适应性和安全性问题,并为农业可持续发展奠定基础。

In order to investigate the effects of different environments and ecological niches on cultivable actinomycetes in sun-cured tobacco,samples were taken from three sites(Longjing,Tumen and Yanji)in the sun-cured tobacco growing areas of Yanbian Prefecture.Pure cultivation methods were used to isolate actinomycetes from tobacco non-rhizosphere soil,rhizosphere soil,roots,stems and leaves.Based on bacterial morphology,16S rDNA partial sequencing,and GenBank-BLAST homology analysis,a total of 11 strains of Streptomyces and Streptococcus were identified,and the number of strains isolated from Yanji sun-cured tobacco samples was higher than that from Longjing and Tumen.Comparative analysis of actinomycetes from different parts of tobacco revealed that the number of isolated actinomycetes ranked from highest to lowest was as follows:roots>stems>leaves.The research results are beneficial for further addressing the adaptability and safety issues in the process of sun-cured tobacco cultivation,and laying the foundation for sustainable agricultural development.