山柰酚联合美罗培南对耐碳氢酶烯类气单胞菌体外作用

目的探讨山柰酚联合美罗培南对耐碳氢酶烯类气单胞菌体外作用效果。方法收集2020年1月至2022年12月嘉兴市中医医院分离的耐碳青霉烯气单胞菌,选取其中的32株进行实验。观察山柰酚与美罗培南联用的体外协同抑菌效应。本实验采用微量肉汤稀释法检测耐碳氢酶烯气单胞菌菌株对山柰酚、美罗培南、山柰酚联合美罗培南的最低抑菌浓度(MIC),计算分级抑制浓度指数(FICI);同时检测对耐碳氢酶烯气单胞菌菌株生长曲线的影响,判断山柰酚与美罗培南是否有协同抑菌作用。结果山柰酚联合美罗培南使用组的MIC低于单药使用组,并可明显抑制耐碳氢酶烯气单胞菌生长。结论山柰酚联合美罗培南体外实验显示具有协同作用,有望改善临床治疗效果及进一步构建联合用药模型用于治疗其他多重耐药感染,但具体机制有待进一步研究。

Objective To investigate the in vitro effect of traditional Chinese medicine kaempferol combined with meropenem on zcarbapenem-resistant Aeromonas,in order to lay a foundation for the treatment and control of infections caused by carbapenem-resistant Aeromonas.Methods 32 strains of carbapenem-resistant aeromonas isolated from Jiaxing Traditional Chinese Medicine Hospital from January 2020 to December 2022 were selected for the experiment.Observe the antibacterial effect of kaempferol combined with meropenem in vitro.This experiment used a micro broth dilution method to detect the minimum inhibitory concentration(MIC)of carbapenem-resistant Aeromonas strains against kaempferol,meropenem,and kaempferol combined with meropenem,and calculated the fractional inhibitory concentration index(FICI);Simultaneously testing the effect on the growth curve of carbapenemresistant Aeromonas strains to determine whether kaempferol and meropenem have combined antibacterial effects.Results The MIC of the combination group of kaempferol and meropenem was lower than that of the single drug group,and it could significantly inhibit the growth of carbapenem-resistant Aeromonas.Conclusion Kaempferol combined with meropenem has a combined effect in vitro experiments,which is expected to improve clinical treatment efficacy and further construct this combined drug model for the treatment of other multidrug-resistant infections.However,the specific mechanism needs further research.