基于单原子铁纳米催化剂的纳米催化疗法对 口腔鳞癌细胞Cal27的作用

Nanocatalytic treatment of oral squamous cell carcinoma Cal 27 cells in vitro based on single-atom iron nanocatalysts

目的:制备单原子铁纳米催化剂(single-atom Fe nanocatalysts,SAF NCs),探讨其体外治疗口腔鳞癌的疗效,为口腔鳞癌治疗提供新策略。方法:用隔离与热解结合方法制备SAF NCs,利用透射电镜和球差电镜观察微观形态,X射线衍射图和元素分布能谱分析其结构和成分,溶液层面采用TMB显色实验和电子自旋共振试验检测催化性能,最后通过CCK-8和流式细胞技术、共聚焦显微镜观察体外处理口腔鳞癌Cal27细胞后的活性变化。采用GraphPad Prism 9软件包对数据进行统计学分析。结果:成功合成和表征SAF NCs,在溶液层面显示出优异的催化特性。细胞实验中,SAF NCs显示出良好的生物相容性,在模拟肿瘤微环境特性的条件下,进行体外催化治疗,Cal27细胞活性低至32.08%。结论:初步实验证明,SAF NCs具有良好的生物催化性能,在体外可有效抑制口腔鳞癌细胞增殖,为口腔鳞癌治疗提供了新策略。

PURPOSE:To prepare single-atom iron nanocatalysts(SAF NCs)and explore its efficacy on oral squamous cell carcinoma Cal 27 cells in vitro,and provide a new strategy for the treatment of oral squamous cell carcinoma.METHODS:SAF NCs were prepared with combination of isolation and pyrolysis,the microscopic characterization was observed by transmission electron microscopy,the morphology and chemical composition were analysed by X-ray diffractograms and elemental distribution energy spectroscopy.The catalytic properties were detected by TMB assay and electron spin resonance test,and finally the changes in the activity of Cal27 cells were observed by CCK-8,flow cytometry and confocal microscopy for in vitro treatment of oral squamous carcinoma,to investigate the therapeutic effect against Cal27 cells.Statistical analysis was performed with GraphPad Prism 9 software package.RESULTS:SAF NCs were successfully synthesized and characterized,which showed excellent catalytic properties at the solution level and good biocompatibility in in vitro cellular level.The viability of Cal27 cell was reduce to 32.08%after in vitro catalytic treatment under conditions mimicking the characteristics of the tumor microenvironment.CONCLUSIONS:Preliminary experiments demonstrated that SAF NCs with good biological properties could effectively inhibit the proliferation of Cal 27 cells in vitro,providing a new strategy for the treatment of oral squamous carcinoma.