不同种桑黄子实体醇提物化学组成及抗氧化活性比较

A comparison of chemical compositions and antioxidant activities of ethanol extracts from basidiomata between different sanghuang species

本研究以产自安徽的鲍姆桑黄、产自浙江和吉林的瓦尼桑黄、产自山东的粗毛纤孔菌以及采自山西的野生桑树桑黄为研究对象,检测桑黄子实体乙醇提取物中的总多酚、三萜及麦角甾醇含量。结果表明:不同物种和不同来源的桑黄子实体中总多酚、三萜、麦角甾醇含量存在差异,其中总多酚含量最高的是产自浙江的瓦尼桑黄(1.99%),三萜含量最高的是产自山东的粗毛纤孔菌(产孢前,1.32%),麦角甾醇含量最高的是产自吉林的瓦尼桑黄(0.19%)。以丙二醛(MDA)为指标比较不同来源桑黄子实体乙醇提取物的抗氧化活性。结果表明,4种桑黄子实体提取物对大鼠肝微粒体脂质过氧化酶MDA的产生均表现出良好的抑制能力,其中浙江桑黄抗氧化活性最佳,MDA抑制率达到96.53%。应用液相色谱-离子阱-飞行时间质谱联用(LC-IT-TOF-MS)技术从桑黄子实体的乙醇提取物中鉴定了19种化合物,其中浙江的瓦尼桑黄、吉林的瓦尼桑黄和安徽的鲍姆桑黄子实体的化合物组成较为相似,主要为hispidin的衍生物;野生的桑树桑黄子实体主要产物也为hispidin衍生物,但结构类型不同于上述3种桑黄;山东的粗毛纤孔菌子实体中则含有大量的hispidin单体,hispidin衍生物含量很少。综上所述,不同来源桑黄的化合物组成、活性产物含量和抗氧化活性存在较大差异,特别是不同种属桑黄样品所含活性产物的种类以及含量差异较大。本研究为桑黄抗氧化产品的开发提供了科学参考。

Content of total polyphenols,triterpenoids and ergosterol of ethanol extracts from basidiomata of different sanghuang species,Sanghuangporus baumii,S.vaninii,S.sanghuang(wild)and Inonotus hispidus from Anhui,Zhejiang,Jilin,Shanxi and Shandong provinces,were compared.The results showed that there were apparent differences in the content of total polyphenols,triterpenoids and ergosterol in basidiomata from different sources.The highest content of total polyphenols was tested in S.vaninii from Zhejiang(1.99%),the highest content of triterpenoids was tested in I.hispidus from Shandong(1.32%),and the highest content of ergosterol was tested in S.vaninii from Jilin(0.19%).Malondialdehyde(MDA)was used as an index to compare the antioxidant activities of different species of sanghuang.The extracts of all tested species showed good inhibitory effects on the production of MDA in rat liver microsomal lipid peroxidase,of which the extract of S.vaninii from Zhejiang had the highest antioxidant activity(96.53%).Nineteen compounds from the aforementioned samples were identified by liquid chromatography-ion trap-time-of-flight mass spectrometry(LC-IT-TOF-MS).Among them,the metabolites of basidiomata of S.vaninii from Zhejiang,S.vaninii from Jilin and S.baumii from Anhui were similar,which were mainly composed of the hispidin derivatives.Sanghuangporus sanghuang(wild)basidiomata mainly contained hispidin derivatives,but the structure types of the derivatives were different from those of aforementioned samples.There was a large quantity of hispidin in basidiomata of I.hispidus from Shandong,and the content of hispidin derivatives was low.In conclusion,the apparent differences in structure types and content of active metabolites as well as their antioxidant activities were observed from different species or localities of sanghuang.This study provides scientific references for the development of antioxidant products of sanghuang species.