虫草素对786-O和ACHN肾癌细胞系增殖、迁移和凋亡机制探究

Mechanisms of proliferation,migration and apoptosis of 786-O and ACHN renal carcinoma cell lines stimulated by cordycepin

探究肾癌细胞系经虫草素给药刺激后,细胞凋亡及迁移的机制。体外培养肾癌786-O细胞系和肾癌ACHN细胞系,采用MTT法、细胞迁移实验、HE染色、免疫荧光染色及蛋白免疫印记法;检验不同浓度虫草素处理肾癌细胞系增殖抑制率、细胞迁移情况、细胞形态变化和细胞核形态差异,检验凋亡相关基因蛋白表达情况,探究虫草素诱导肾癌细胞的凋亡机制。随着浓度剂量提高,虫草素能够显著促进肾癌细胞系凋亡,抑制迁移。形态学研究表明,HE染色观察发现癌细胞数量明显降低,并且细胞核明显变大。免疫荧光染色发现MMP-2、MMP-9和BCL-2蛋白表达显著降低,Bax、Casepase-3和Casepase-7蛋白表达显著增加,肾癌细胞可通过AKT/mTOR信号通路诱导肾癌细胞凋亡。

The mechanism of apoptosis and migration of renal cancer cell lines after stimulation by cordycepin administration was explored.Kidney cancer 786-O cell line and kidney cancer ACHN cell line were cultured in vitro,and MTT method,cell migration experiment,HE staining,immunofluorescence staining,and protein immunoimprinting were used to examine the inhibition rate of proliferation,cell migration,cell morphology and nuclear morphology of renal cancer cell lines under different concentration of cordycepin administration.The expression of apoptosis-related gene proteins was investigated,and the mechanism of apoptosis induced by cordycepin in renal cancer cells was explored.With the increase of concentration and dose,cordycepin can significantly promote apoptosis and inhibit migration of renal cancer cell lines.Morphological and HE staining observation showed that the number of cancer cells was significantly reduced,and the nucleus was significantly enlarged.Immunofluorescence staining showed that the expression of MMP-2,MMP-9 and BCL-2 proteins decreased significantly,the expression of Bax,Casepase-3 and Casepase-7 proteins increased significantly,and the apoptosis renal cancer cells could be induced through the AKT/mTOR signaling pathway.