不同时期独活不同部位中香豆素类成分积累规律研究

Accumulation Rule of Coumarins in Different Parts of Angelica pubescens in Different Periods

目的:建立采用气相色谱-质谱联用(GC-MS)法分析独活中香豆素类成分的新方法,明确不同时期、不同部位独活中香豆素类成分含量的动态变化规律。方法:采用GC-MS技术对不同时期独活根、茎、叶中主要成分蛇床子素、花椒毒素、二氢欧山芹醇当归酸酯和二氢欧山芹素等香豆素类化合物进行相对定量测定,分析不同时期、不同部位独活中香豆素类成分含量的动态变化。结果:独活不同部位在不同时期的化合物种类数不同,根于10月收获期时化合物种类数达到最多,茎叶于8月种类数达到最大,根、茎、叶中化合物种类数分别为59种、45种、44种,共有成分有38种,且成分种类间存在差异。结论:首次建立了采用GC-MS法分析独活中香豆素类成分的新方法,研究了香豆素类成分的积累规律,发现独活不同部位化学成分种类和含量存在差异性。此项研究为独活药材的质量控制及高效利用提供一定的科学依据。

Objective:To establish a new method using gas chromatography-mass spectrometry(GC-MS) to analyze coumarins in Angelica pubescens and to clarify the dynamic changes in the content of coumarins in different parts of A.pubescens in different periods.Methods:GC-MS technology was used to determine the main components of coumarins such as cnidium,ligustilide,dihydroalsyl angelica,and dihydroalcein in the roots,stems,and leaves of A.pubescens in different periods,and analyze the dynamic changes in the content of coumarins in different parts of A.pubescens in different periods.Results:The number of compound types in different parts of A.pubescens varied in different periods.The number of compound types in the roots was the highest in October harvest period,while in the stems and leaves,it reached the maximum in August with 59,45,and 44 compound types in roots,stems,and leaves respectively.There were 38 common components,and differences were observed among the types of components.Conclusion:A new method for analyzing coumarins in A.pubescens using GC-MS was established for the first time.The accumulation pattern of coumarins was studied,and differences were found in the types and contents of chemical components in different parts of A.pubescens.This study provides a scientific basis for the quality control and efficient utilization of A.pubescens medicinal materials.