RASA4表达失活促进鼻咽癌增殖的机制研究

Mechanism of RASA4 expression inactivation promoting proliferation of nasopharyngeal carcinoma

目的:探究RASA4基因在鼻咽癌(NPC)中的表达情况,研究RASA4基因对影响NPC增殖和转移的影响及分子机制。方法:利用GEO数据库进行NPC中RASA4转录水平的Meta分析。采用实时荧光定量PCR(RT-qPCR)和western blotting法检测NPC细胞中RASA4 mRNA及蛋白表达水平,免疫组织化学染色法检测RASA4在NPC组织中的蛋白表达及定位情况,CCK-8及平板克隆实验评估RASA4对NPC细胞增殖能力的影响。结果:基于GEO数据的生物信息学分析显示,RASA4在NPC中的m RNA表达显著下调。RT-qPCR和western blotting显示NPC细胞中RASA4 mRNA及蛋白表达下调。外源性过表达RASA4基因可抑制NPC细胞增殖。结论:RASA4可能是NPC潜在的抑制基因,研究其失活机制及其功能对于NPC的发生机制和治疗具有重要意义。

Objective:To explore the expression of RASA4 gene in nasopharyngeal carcinoma(NPC),and to investigate the influence and molecular mechanism of RASA4 gene affecting the proliferation of NPC.Methods:Meta-analysis of RASA4 transcription levels in NPC was performed using GEO database.RASA4 mRNA and protein expression levels in NPC cell lines were detected by reverse transcription-quantitative PCR(RT-qPCR)and western blotting.RASA4 protein expression and localization in NPC tissues were detected using immunohistochemistry(IHC).The impact of RASA4 on cell proliferation was evaluated through cell counting kit-8(CCK-8) assay and colony formation assay.Results:Bioinformatics analysis based on GEO data showed that RASA4mRNA expression was significantly down-regulated in NPC.RT-qPCR and western blotting results indicated that RASA4 was down-regulated at both m RNA and protein expression in NPC cells.Exogenous overexpression of the RASA4 gene could inhibit the cell proliferation of NPC.Conclusion:RASA4 may serve as a potential suppressor gene in NPC,and investigating its inactivation mechanism and function is of significant importance for the pathogenesis and treatment of NPC.