核酸检测法与ELISA检测法对血液标本中丙肝病毒检测的对比分析

Comparative Analysis of HCV Detection in Blood Samples by Nucleic Acid Testing and ELISA Testing

目的:分析核酸检测法与酶联免疫检测法(ELISA)对血液标本中丙肝病毒检测的应用情况。方法:对1200例血液样本分别采用核酸检测、ELISA法检测,对比两种检测方法检出阳性率以及对丙型肝炎病毒感染诊断价值。结果:以HCVRNA检测结果为金标准,丙型肝炎病毒检出阳性率为4.00%,其中联合检测检出阳性率3.92%,均高于核酸检测法的2.67%、ELISA法的2.50%,差异有统计学意义(P<0.05)。联合检测检出价值均高于核酸检测法、ELISA检测法(P<0.05)。结论:核酸检测法与ELISA检测法用于血液标本内丙肝病毒检测均有一定价值,其中核酸检测法检测阳性率略微高于ELISA检测法,但为了提高疾病筛查率,要联合以上方法共同测定。

Objective:To analyze the application of nucleic acid detection and enzyme-linked immunodetection(ELISA)for HCV detection in blood samples.Methods:1200 blood samples were tested using nucleic acid testing and ELISA,and the positive rates of the two methods were compared,as well as their diagnostic value for hepatitis C virus infection.Results:Using the HCV RNA detection results as the gold standard,the positive rate of hepatitis C virus detection was 4.00%,with a combined detection positive rate of 3.92%,both higher than the 2.67%of nucleic acid detection and 2.50%of ELISA,with statistically significant differences(P<0.05).The detection value of joint testing is higher than that of nucleic acid testing and ELISA testing(P<0.05).Conclusion:Both nucleic acid testing and ELISA testing have certain value in detecting hepatitis C virus in blood samples.The positive rate of nucleic acid testing is slightly higher than that of ELISA testing.However,in order to improve the disease screening rate,it is necessary to combine the above methods for testing.