基于网络药理学探讨健脾温阳方治疗原发性早泄的作用机制

Exploration of the mechanism of action of Jianpi Wenyang prescription in treating primary premature ejaculation based on network pharmacology

目的基于网络药理学分析健脾温阳方治疗原发性早泄(PPE)的潜在作用机制。方法使用中药系统药理学数据库与分析平台(TCMSP)、中药分子机制的生物信息学分析工具(BATMAN-TCM)平台采集健脾温阳方中所有药物的化学活性成分,包括对应的主要目标靶点;使用在Genecards数据库、Drugbank数据库采集PPE的有关作用目标靶点;将有效成分靶点和病痛靶点取交集,获取和有效成分和病痛相联系的交集靶点,绘制韦恩图;使用Cytoscape软件将交集最终结果绘图设计成“活性成分-靶点”网络分布图;使用STRING数据库绘制蛋白质相互作用(PPI)网络分布图;使用注释、可视化和集成发现的数据库(DAVID)展开基因本体(GO)和京都基因与基因组百科全书(KEGG)研究分析。结果网络药理学结果显示,健脾温阳方治疗PPE的关键化学成分为槲皮素,靶点基因为Jun、MAPK3、MAPK1、ESR1、FOS等,关键通路是PI3KAkt信号通路和cAMP信号通路。结论利用网络药理学方法探究健脾温阳方在PPE治疗过程中的主要作用机制和共同作用靶点等,为揭示其化学活性有效成分提供了理论参考依据。

Objective To analyze the potential mechanism of action of Jianpi Wenyang prescription in treating primary premature ejaculation(PPE)based on network pharmacology.Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and the Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine(BATMAN-TCM)platform were used to collect the chemical active ingredients of all drugs in Jianpi Wenyang prescription,including corresponding main targets.The relevant action targets of PPE were collected using Genecards and Drugbank databases.The effective component targets were intersected with the pain targets,the intersection targets associated with the effective component and the pain were obtained,and a Venn diagram was drawn and designed.Cytoscape software was used to plot and design the final intersection results into a"active components-targets"network distribution map.A protein-protein interactions(PPI)network distribution map was created using the STRING database.The gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)research analysis were conducted using the database for annotation,visualization and integrated discovery(DAVID).Results The network pharmacology results of this study showed that the critical chemical component of Jianpi Wenyang prescription in treating PPE is quercetin,and the target genes are Jun,MAPK3,MAPK1,ESR1,FOS,etc.The critical pathways are the PI3K-Akt signaling pathway and the cAMP signaling pathway.Conclusion The use of network pharmacology methods to explore the main mechanism of action and common action targets of Jianpi Wenyang prescription in the treatment of PPE provides a theoretical reference for revealing its chemically active effective components.