罗汉松种子总黄酮的抗炎作用研究

Study on anti-inflammatory effect of total flavonoids from Podocarpus macrophyllus seeds

目的研究罗汉松种子总黄酮的抗炎作用。方法构建角叉菜胶致小鼠足肿胀、二甲苯致小鼠耳肿胀、脂多糖(LPS)诱导小鼠致炎三种急性致炎实验模型,各模型随机分为空白对照组、阿司匹林组(阿司匹林200 mg/kg)、罗汉松种子总黄酮低、高剂量组(60、180 mg/kg),后2个模型增设模型组,每组各10只,分别灌胃给药,1次/d,连续7 d。角叉菜胶致小鼠足肿胀实验模型中,小鼠右后足注射角叉菜胶致炎,左足不注射,取小鼠左右两足称重,计算足肿胀度和肿胀抑制率。二甲苯致小鼠耳肿胀实验模型中,小鼠右耳涂抹二甲苯致炎,左耳不涂抹,取小鼠左右两耳片称重,计算耳肿胀度和肿胀抑制率;再取血,用酶联免疫吸附(ELISA)法检测血清中白细胞介素-1β(IL-1β)和白细胞介素-8(IL-8)含量。LPS诱导小鼠炎症模型中,除空白对照组外,其余各组小鼠腹腔注射LPS致炎,取血,用ELISA法检测血清中肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-10(IL-10)含量。结果罗汉松种子总黄酮低、高剂量组对角叉菜胶致小鼠足肿胀的抑制率分别为12.67%和14.49%,对二甲苯致小鼠耳肿胀的抑制率分别为15.88%和47.65%,对小鼠血清IL-1β和IL-8影响不大(P>0.05),但能明显降低TNF-α、IL-6含量和升高IL-10含量(P<0.05)。结论罗汉松种子总黄酮能抑制小鼠足、耳肿胀,具有一定抗炎作用,可能与降低血清TNF-α、IL-6含量和升高IL-10含量有关。

Objective To study the anti-inflammatory effect of total flavonoids from Podocarpus macrophyllus seeds.Methods Three acute inflammatory experimental models were established,namely,carrageenin-induced mouse foot swelling,xylene-induced mouse ear swelling,and lipopolysaccharide(LPS)-induced mouse inflammation.Each model was randomly divided into a blank control group,a aspirin group(200 mg/kg of aspirin),a low-dose group(60 mg/kg of total flavonoids from the seeds of Podocarpus macrophyllus),and a high-dose group(180 mg/kg of total flavonoids from the seeds of Podocarpus macrophyllus).The last two were added to a model group with 10 mice in each group.The drug was administered by gavage once a day for 7 consecutive days.In the experimental model of carrageenan-induced mouse foot swelling,the right hind foot of the mouse was injected with carrageenan to cause inflammation,while the left foot was not injected.The left and right feet of the mouse were weighed and the degree of foot swelling and swelling inhibition rate were calculated.In the xylene-induced mouse ear swelling experimental model,the right ear of the mous e was inflamed by applying xylene,while the left ear was not applie d.The left and right ear slices of the mouse were weighed,and the degree of ear swelling and swelling inhibition rate were calculated;took blood again and used enzyme-linked immunosorbent assay(ELISA)to detect the levels of interleukin-1β(IL-1β)and interleukin-8(IL-8)in serum.In the LPS-induced mouse inflammation model,except for the blank control group,all other groups of mice were intraperitoneally injected with LPS to induce inflammation.Blood was collected and levels of tumor necrosis factors in serum such as tumor necrosis factor α(TNF-α),interleukin-6(IL-6)and interleukin-10(IL-10)levels were detected by ELISA.Results The inhibition rates of the low and high-dose groups of total flavonoids from Podocarpus macrophyllus seeds on the foot swelling of mice induced by carrageenin were 12.67% and 14.49%,respectively,and the inhibition rates on the ear swelling of mice induced by xylene were 15.88% and 47.65%,respectively.It had little effect on IL-1β and IL-8(P>0.05),but could significantly reduce TNF-α and IL-6 levels,and increase IL-10 level(P<0.05).Conclusion The total flavonoids of Podocarpus macrophyllus seeds can inhibit the swelling of the feet and ears of mice and have certain anti-inflammatory effects,which may be related to the reduction of serum TNF-α and IL-6 levels,and increase in IL-10 level.