外周血液中脂筏标记蛋白表达水平与重性抑郁障碍全病程相关性的研究

Study on correlation between the expression level of lipid raft marker protein in peripheral blood and the whole course of major depressive disorder

目的探讨外周血液中脂筏标记蛋白(FLOT)1表达水平与重性抑郁障碍(MDD)全病程的相关性,以及是否能起到预测MDD复发与难治性MDD的作用。方法选取福建省福州神经精神病防治院2022年9月至2023年8月收治的100例MDD患者为试验组,以同期50名健康体检者为对照组。分别采取不同时期(急性期、巩固期、稳定期)MDD患者及对照组的外周血样本,采用实时荧光定量PCR(qRT-PCR)法测定外周血单核细胞FLOT1表达水平,分析FLOT1表达水平变化与生活事件(主要为负性事件)的相关性。结果试验组FLOT1 mRNA相对表达量高于对照组,差异有统计学意义(P<0.05);与治疗前比较,试验组治疗后的FLOT1 mRNA相对表达量明显下降,差异有统计学意义(P<0.05);试验组急性期的FLOT1 mRNA相对表达量明显高于巩固期与稳定期,差异有统计学意义(P<0.05)。试验组急性期汉密顿抑郁量表-17(HAMD-17)评分均高于巩固期、稳定期,差异有统计学意义(P<0.05);稳定期与巩固期比较,差异无统计学意义(P>0.05)。FLOT1 mRNA相对表达量与MDD患者不同时期的HAMD-17评分呈正相关(P<0.05),FLOT1 mRNA相对表达量与负性事件刺激量、生活事件应激总分呈正相关(P<0.05)。结论外周血液中FLOT1表达水平与MDD全病程有相关性,可能是MDD发病的一种生物学标志物。

Objective To explore the correlation between the expression level of lipid rafts marker protein flotillin(FLOT)1 in peripheral blood and the whole course of major depressive disorder(MDD),and whether it can predict the MDD recurrence and refractory MDD.Methods A total of 100 MDD patients admitted to Fuzhou Neuropsychiatric Hospital of Fujian Province from September 2022 to August 2023 were selected as the experimental group and 50 healthy people as the control group.The peripheral blood samples were taken from the patients in different stages of MDD(acute stage,consolidation stage and stable stage)and the control group,and the expression level of FLOT1 in peripheral blood mononuclear cells was measured by real-time fluorescence quantitative PCR(qRT-PCR),and the correlation between the change of FLOT1 expression level and life events(mainly negative events)was analyzed.Results The relative expression of FLOT1 mRNA in the experimental group was higher than that in the control group,with statistically significant difference(P<0.05).The relative expression of FLOT1 mRNA in the experimental group after treatment was lower than that before treatment,with statistically significant difference(P<0.05).The relative expression of FLOT1 mRNA in the acute phase of the experimental group was higher than that in the consolidation phase and the stable phase,with statistically significant difference(P<0.05).T he score of H amilton depression scale-17(HAMD-17)in the experim ental group was higher than that in the consolidation period and the stable period,with statistically significant difference(P<0.05).There was no statistical difference in HAMD score between the stable period and the consolidation period(P>0.05).The relative expression of FLOT1 mRNA was positively correlated with the HAMD-17 score of the different stages of MDD patients(P<0.05),and the relative expression of FLOT1 mRNA was positively correlated with the amount of stimulus of negative events and the total stress score of life events(P<0.05).Conclusion The expression level of FLOT1 in peripheral blood is related to the whole course of MDD,which may be a biological marker of MDD.