DNA条形码技术融入普通昆虫学实验教学

Integrating DNA Barcoding into Experimental Teaching of General Entomology

为了提升普通昆虫学实验教学质量,以DNA条形码技术为例,尝试将最新科研成果和技术与普通昆虫学实验教学进行融合。介绍了植物保护专业学生掌握DNA条形码技术的必要性,分析了在普通昆虫学实验教学中开展DNA条形码实验的可行性,在样品保存、DNA提取、PCR扩增、电泳检测、测序和分析等方面优化了以COI基因序列作为DNA条形码开展分子鉴定的具体流程,设计了开展DNA条形码专题实验或者将其与传统分类实验结合的实施和考核方案。DNA条形码技术的融入改变了普通昆虫学实验传统教学模式,也为探索创新性实验教学提供了思路。

In order to improve the quality of experimental teaching in general entomology and cultivate more innovative talents for the major of plant protection under the background of new agricultural sciences,taking DNA barcoding as an example,the latest scientific research achievement and technology are integrated with the experimental teaching.Herein,the necessity for students majoring in plant protection to master DNA barcoding is introduced,the feasibility of carrying out DNA barcode experiment in the experimental teaching of General Entomology is analyzed,the specific process of molecular identification using COI gene sequences as DNA barcodes is optimized in sample preservation,DNA extraction,PCR amplification,electrophoresis detection,sequencing and analysis.In addition,the implementation and assessment plan for conducting the topical experiment of DNA barcoding or combining it with traditional taxonomy experiments is designed.The integration of DNA barcoding changes the traditional experimental teaching mode of general entomology,and provides ideas for exploring innovative experimental teaching.