饲用油脂总能测定条件优化研究

Optimization Study of Gross Energy Measurement Conditions for Feed Fat and Oil

试验选用氧弹量热法进行饲用油脂总能测定条件优化研究。选用豆油、调和油作为试验材料,研究是否添加基质对总能测定结果的影响,进行检测方法优化;选用11种油脂作为试验材料,验证不同试样质量(0.4、0.6、0.8、1.0、1.2 g)对测定结果的影响;选用5种油脂作为试验材料,比对不同仪器对测定结果的影响。结果表明:测定豆油总能,添加基质后测定结果变异系数大,重复性差(P<0.05),不添加基质重复性好(P>0.05);不添加基质方法进行日内、日间试验,测定结果差异不显著(P>0.05),测定结果稳定性好(P<0.05)。不同试样质量对豆油、调和油、椰子油、月桂酸、鱼油、鸡油1#、鸡油2#、猪油1#、猪油2#等9种油脂总能测定结果差异不显著(P>0.05),对棕榈油总能测定结果表现为差异显著(P<0.05),对磷脂总能测定结果表现为差异极显著(P<0.001),试样质量适宜范围为0.6~0.8 g。两种氧弹量热仪测定椰子油、棕榈油、豆油、月桂酸、磷脂5种油脂相同试样质量,测定总能结果差异不显著(P>0.05)。本试验条件下,油脂测定试验无需添加基质,该方法测定总能结果稳定、重复性好。不同试样质量对豆油、调和油、椰子油、月桂酸、鱼油、鸡油1#、鸡油2#、猪油1#、猪油2#等9种油脂表现为差异不显著,适宜试样质量为0.4~1.0 g;对棕榈油表现为差异显著,适宜试样质量为0.6~1.0 g;对磷脂表现为差异极显著,适宜试样质量为0.8~1.0 g。用两种氧弹量热仪测定油脂总能结果无明显差异。

In this experiment,oxygen bomb calorimetry was used to optimize the determination conditions of gross energy of feed fat and oil.Soybean oil and blended oil were selected as experimental materials to study the effect of matrix addition on the results of gross energy determination and optimize the detection method.Eleven types of feed fats and oils were selected as experimental materials to verify the influence of different sample masses(0.4,0.6,0.8,1.0,1.2 g)on the determination results,while five types of fats and oils were se‐lected to compare the influence of different instruments on the determination results.The results showed that matrix addition resulted in a large coefficient of variation and poor repeatability when determining the gross energy of soybean oil(P<0.05),while no matrix group showed good repeatability(P>0.05).The intra-day and inter-day measurement with no matrix showed insignificant differences in determination results,which indicated good stability of the results(P>0.05).The different sample masses had no significant effect on the determination results of nine types of feed fats and oils,such as soybean oil,blended oil,coconut oil,lauric acid,fish oil,chicken fat 1#,chicken fat 2#,lard 1#,and lard 2#(P>0.05).However,there was a significant difference in gross energy determination results for palm oil(P<0.05)and for lecithin(P<0.001).The suitable sample mass range was 0.6 g to 0.8 g.No significant differences were observed in the gross energy determination results of coconut oil,palm oil,soybean oil,lauric acid,and lecithin when using two different oxygen bomb calorimeters with the same sample mass(P>0.05).Under the conditions of this experiment,there is no need to add a matrix for oil gross energy determination experiments.The method used in this experiment has a stable gross energy determination result and good repeatability.The influence of different sample masses on gross energy results is insignificant for nine types of fats and oils(such as soybean),and the suitable sample mass range was 0.4 g to 1.0 g.Meanwhile,palm oil shows significant difference and lecithin shows extremely difference while the suitable sample mass range was 0.8 g to 1.0 g.There are no significant differences in gross energy results between two different oxygen bomb calorimeters.