上海交通大学实验动物中心公共细胞培养平台管理经验探讨

Discussion on Management Experience of Public Cell Culture Platform in Laboratory Animal Center of Shanghai Jiao Tong University

公共细胞培养平台作为实验动物设施中一个重要的配套设施,是实验动物肿瘤疾病模型以及转基因动物制备等科学研究的重要支撑。实验动物中心搭建公共细胞培养实验平台,能充分整合实验动物和细胞培养资源,更为高效、合理地配置相关科研资源,便于科研人员更好地开展研究。实验动物中心公共细胞培养平台内培养的细胞大部分用于动物实验,细胞如果存在污染或者质量问题,不但影响实验结果,还会影响实验动物健康,造成实验动物的微生物感染,严重者甚至会扩散污染整个动物饲养设施。因此实验动物中心的公共细胞培养实验室在细胞质量把控上相比常规的细胞培养室更为严格。本文以上海交通大学实验动物中心公共细胞培养平台为例,从设施维护与管理、人员管理、细胞生物风险质控管理等三个主要控制点对公共细胞培养室的管理经验进行了探讨,以期为国内其他实验动物机构的公共细胞培养实验室管理提供有益借鉴。

Public cell culture platform is an important facility in laboratory animal facilities,providing essential support for scientific research such as the development of animal tumor disease models and transgenic animals.By establishing a public cell culture experimental platform,laboratory animal centers can effectively integrate experimental animals and cell culture resources,optimizing the allocation of scientific research resources to facilitate better research outcomes.The majority of cells cultured in these platforms are used for animal experiments.Contamination or quality issues in these cells not only affect experimental results but also jeopardize the health of experimental animals,potentially leading to microbial infections and contamination of entire animal facilities.Therefore,public cell culture laboratories within experimental animal facilities impose stricter quality control measures than conventional cell culture rooms.This study takes the public cell culture platform at the Laboratory Animal Center of Shanghai Jiao Tong University as a case study to discuss management experiences,focusing on facility maintenance and management,personnel management and quality control of cell biological risk.The aim is to provide useful reference for the management of public cell culture laboratories in experimental animal facilities and other institutions.