胸腔积液沉渣包埋组织TTF-1、Galectin-3的表达水平与肺腺癌恶性生物学行为间的关系

Relationship between the expression levels of TTF-1 and Galectin-3 in sediment embedded tissues in pleural effusion and malignant biological behavior of lung adenocarcinoma

目的探讨胸腔积液沉渣包埋组织甲状腺转录因子1(TTF-1)、半乳糖凝集素-3(Galectin-3)的表达水平与肺腺癌恶性生物学行为间的关系。方法选取2020年10月至2023年3月在该院就诊的肺腺癌患者163例,根据分化程度分为高分化组(26例)、中分化组(78例)、低分化组(59例)。对比3组胸腔积液沉渣包埋组织TTF-1、Galectin-3及恶性生物学行为相关基因(p16基因mRNA)表达水平,Spearman秩相关系数分析胸腔积液沉渣包埋组织TTF-1、Galectin-3与p16基因mRNA表达水平的相关性,多元线性回归分析胸腔积液沉渣包埋组织TTF-1、Galectin-3与p16基因mRNA表达水平间的依存关系,限制性立方样条图分析胸腔积液沉渣包埋组织TTF-1、Galectin-3与p16基因mRNA表达水平间的剂量-效应关系。结果低分化组胸腔积液沉渣包埋组织TTF-1、Galectin-3表达水平高于中分化组、高分化组,p16基因mRNA表达水平低于中分化组、高分化组,中分化组胸腔积液沉渣包埋组织TTF-1、Galectin-3表达水平高于高分化组,p16基因mRNA表达水平低于高分化组,差异有统计学意义(P<0.05);胸腔积液沉渣包埋组织TTF-1(r=-0.752,P<0.001)、Galectin-3(r=-0.811,P<0.001)表达水平与p16基因mRNA表达水平呈负相关;胸腔积液沉渣包埋组织Galectin-3表达水平与p16基因mRNA表达水平间无线性依存关系(P>0.05),胸腔积液沉渣包埋组织TTF-1表达水平与p16基因mRNA表达水平间有线性依存关系(P=0.049);当胸腔积液沉渣包埋组织TTF-1表达水平<4分、胸腔积液沉渣包埋组织Galectin-3表达水平<3分时,对p16基因mRNA表达作用最显著。结论胸腔积液沉渣包埋组织TTF-1、Galectin-3表达水平与肺腺癌恶性生物学行为关系密切,早期检测对临床实现精准干预避免病情恶化具有重要意义。

Objective To investigate the relationship between the expression levels of thyroid transcription factor 1(TTF-1)and Galectin-3(Galectin-3)in sediment embedded tissues in pleural effusion and the malignant biological behavior of lung adenocarcinoma.Methods A total of 163 patients with lung adenocarcinoma in the hospital from October 2020 to March 2023 were selected and divided into highly differentiated group(26 cases),moderately differentiated group(78 cases)and poorly differentiated group(59 cases)according to the degree of differentiation.The expression levels of TTF-1,Galectin-3 and p16 mRNA in sediment embedded tissues in pleural effusion in the three groups were compared.Spearman rank correlation coefficient was used to analyze the correlation between TTF-1,Galectin-3 expression and p16 mRNA expression in sediment embedded tissues in pleural effusion.Multiple linear regression analysis was performed to determine the dependence of TTF-1,Galectin-3 expression and p16 mRNA expression in sediment embedded tissues in pleural effusion.The dose-effect relationship between the expression of TTF-1,Galectin-3 and p16 mRNA in sediment embedded tissues in pleural effusion was analyzed by restricted cubic spline diagram.Results The expression levels of TTF-1 and Galectin-3 in the low-differentiation group were higher than those in the medium-differentiation group and the high-differentiation group,the expression of p16 gene mRNA was lower than that in the medium-differentiation group and the high-differentiation group,and the expression levels of TTF-1 and Galectin-3 in the medium-differentiation group were higher than that in the high-differentiation group,and the expression levels of p16 gene mRNA was lower than that in highly differentiated group,and the differences were statistically significant(P<0.05).The expression levels of TTF-1(r=-0.752,P<0.001)and Galectin-3(r=-0.811,P<0.001)were negatively correlated with p16 mRNA expression in pleural sludge embedding tissues.There was no significant linear dependence between Galectin-3 expression and p16 gene mRNA expression levels in sediment embedded tissues in pleural effusion(P>0.05),but there was a linear dependence between TTF-1 expression and p16 gene mRNA expression levels in sediment embedded tissues in pleural effusion(P=0.049).When the expression level of TTF-1 in sediment embedded tissues in pleural effusion was less than 4 points,and the expression level of Galectin-3 in sediment embedded tissues in pleural effusion was less than 3 points,the effect on p16 mRNA expression was the most significant.Conclusion The expression levels of TTF-1 and Galectin-3 in sediment embedded tissues in pleural effusion is closely related to the malignant biological behavior of lung adenocarcinoma.Early detection is of great significance for clinical intervention to avoid disease deterioration.