骨关节炎患者血清HDAC3表达水平及临床意义

Expression level and clincial significance of serum HDAC3 in patients with osteoarthritis

目的探究骨关节炎(OA)患者血清组蛋白去乙酰化酶3(HDAC3)表达水平及临床意义。方法选取该院2021年10月至2023年1月收治的128例OA患者为研究对象(OA组),根据Kellgren-Lawrence(K-L)评分系统将OA患者分为Ⅱ级组(n=52)、Ⅲ级组(n=46)、Ⅳ级组(n=30)3个亚组,另选取同期进行健康体检的体检健康者128例为对照组。采用酶联免疫吸附试验(ELISA)测定所有受试者血清HDAC3表达水平;比较不同组间OA患者血清HDAC3表达水平及OA症状评分;Spearman相关性分析OA患者血清HDAC3表达水平与K-L分级、OA症状评分[疼痛评分、僵硬评分、关节功能评分及骨关节炎指数(WOMAC)总分]之间的关系;受试者工作特征(ROC)曲线分析血清HDAC3对OA的预测价值。结果与对照组相比,OA组患者血清HDAC3表达水平显著升高,差异有统计学意义(P<0.05);不同K-L分级OA患者血清HDAC3表达水平及症状评分比较,差异有统计学意义(P<0.05),随着K-L分级的增加,OA患者血清HDAC3表达水平、疼痛评分、僵硬评分、关节功能评分及WOMAC总分均显著增加,差异有统计学意义(P<0.05);Spearman相关性分析显示,血清HDAC3表达水平与K-L分级呈显著正相关(r=0.514,P<0.05),HDAC3表达水平与疼痛评分、僵硬评分、关节功能评分及WOMAC总分均呈显著正相关(P<0.05);ROC曲线结果显示,血清HDAC3诊断OA的曲线下面积为0.857(95%CI:0.809~0.905),灵敏度为81.3%,特异度为70.4%。结论OA患者血清HDAC3表达水平显著升高,且随着K-L分级的增加,血清HDAC3表达水平逐渐增加,HDAC3是预测OA的有效指标。

Objective To investigate the expression level and clinical significance of serum histone deacetylase 3(HDAC3)in patients with osteoarthritis(OA).Methods A total of 128 patients with OA admitted to the hospital from October 2021 to January 2023 were selected as the study objects(OA group).According to the Kellgren-Lawrence(K-L)scoring system,OA patients were grouped into three subgroups:gradeⅡgroup(n=52),gradeⅢgroup(n=46),and gradeⅣgroup(n=30).Another 128 heatlhy individuals who underwent physical examinations were regarded as the control group.Enzyme linked immunosorbent assay(ELISA)was applied to compare and measure the serum HDAC3 expression levels of all study objects.The serum HDAC3 expression levels and OA symptom scores were compared among different groups.Spearman correlation was applied to analyze the relationship between serum HDAC3 expression level and K-L grade,and OA symptom scores[pain score,stiffness score,joint function score,and total Western Ontario and McMaster Universities(WOMAC)osteoarthritis index score].Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum HDAC3 for OA.Results Compared with the control group,the serum HDAC3 expression level in the OA group was obviously increased,and the difference was statistically significant(P<0.05).There were obvious differences in serum HDAC3 expression level and symptom score among OA patients with different K-L grades(P<0.05).As the K-L grades of the disease increased,the serum HDAC3 expression level,pain score,stiffness score,joint function score,and total WOMAC score of OA patients all obviously increased,and the differences were statistically significant(P<0.05).Spearman correlation analysis showed that serum HDAC3 expression level was obviously positively correlated with the K-L grades(r=0.514,P<0.05),and HDAC3 expression level was obviously positively correlated with pain score,stiffness score,joint function score,and total WOMAC score(P<0.05).ROC curve results showed that the area under the curve of serum HDAC3 in diagnosing OA was 0.857(95%CI:0.809-0.905),with a sensitivity of 81.3%and a specificity of 70.4%.Conclusion The serum HDAC3 expression level in OA patients obviously increases,and with the increase of K-L grade,the serum HDAC3 expression level gradually increases.HDAC3 is an effective indicator for predicting OA.