一株高效解钾菌的筛选、鉴定及培养条件的优化

Screening,identification and culture conditions optimization of a highly efficient potassium-solubilizing bacteria

以黑土水稻田中解钾菌为研究目标,共分离筛选出5株解钾菌,通过其形态特征以及测定菌株解钾能力筛选出1株高效解钾菌株S1。利用16S rDNA鉴定S1的种属,并通过单因素试验和正交试验优化该菌株的培养条件。结果表明,分离筛选得到的5株解钾菌均能有效分解钾长石,菌株S1解钾能力最强,解钾率为57.03%,发酵液中解钾量为3.852 mg/L,为最优解钾菌。初步鉴定解钾菌S1为伯克霍尔德菌(Burkholderia ambifaria)。经单因素试验和正交试验优化后的培养组分为碳源1.0%甘露醇、氮源1.0%蛋白胨、无机盐0.5%K_(2)HPO_(4),培养条件为温度30℃、时间48 h、培养基装液量(培养瓶250 mL)80 mL、初始pH 6.5、接种量5.0%。

Five strains of potassium-solubilizing bacteria were isolated and screened from black soil paddy field.One strain(S1)with high efficiency of potassium-solubilizing was screened by its morphological characteristics and potassium-solubilizing ability.The spe⁃cies of S1 was identified by 16S rDNA,and the culture conditions of the strain were optimized by the single factor test and orthogonal test.The results showed that the five strains of potassium-solubilizing bacteria were all able to decompose potassium feldspar effective⁃ly.Strain S1 had the highest potassium-solubilizing ability with a potassium-solubilizing rate of 57.03%,and the content of potassi⁃um-solubilizing in the fermentation broth was 3.852 mg/L,which was the best potassium-solubilizing bacteria.The potassium-solubi⁃lizing strain S1 was identified as Burkholderia ambifaria.The optimized culture conditions were carbon source 1.0%mannitol,nitrogen source 1.0%peptone,inorganic salt 0.5%K_(2)HPO_(4),culture temperature 30℃,culture time 48 h,liquid volume 80 mL/250 mL,ini⁃tial pH 6.5,and inoculation volume 5.0%.