蛋白核小球藻产油率的影响因素

Influencing factors of oil production rate of Chlorella pyrenoidosa

采用BG-11为基础培养基,通过添加、替换不同碳源和氮源、改变碳源和氮源比例、去除磷元素或硫元素及额外添加植物生长调节剂建立不同培养体系,进行蛋白核小球藻(Chlorella pyrenoidosa)培养,考察各因素对蛋白核小球藻生长情况及产油率的影响。结果表明,无论是自养体系还是异养体系,蛋白核小球藻生长量和产油率均比普通小球藻(Chlorella vulgaris)更优,异养体系下2种小球藻产油率分别为20.14%和18.66%;葡萄糖为碳源时蛋白核小球藻生长情况最好且产油率最高,产油率为26.40%;氮源为尿素时蛋白核小球藻有最大产油率,为32.70%;碳氮比为30∶1时蛋白核小球藻产油率最高,为34.42%;硫元素缺乏时蛋白核小球藻产油率比对照提高28.54个百分点;1.5 mg/L茉莉酸干扰下,蛋白核小球藻产油率最高,为40.42%。由此得出,蛋白核小球藻生长量和产油率都比普通小球藻更优;葡萄糖为最优碳源;尿素为最优氮源;碳氮比为30∶1时产油率最高;硫元素缺乏时对蛋白核小球藻影响更大;1.5 mg/L茉莉酸干扰下,蛋白核小球藻产油率最高。

BG-11 was used as the basic medium to establish different culture systems for Chlorella pyrenoidosa culture by adding and replacing different carbon and nitrogen sources,changing the proportion of carbon and nitrogen sources,removing phosphorus or sul⁃fur and adding additional plant growth regulator,and the effects of various factors on the growth and oil production rate of Chlorella py⁃renoidosa were investigated.The results showed that the growth and oil production rate of Chlorella pyrenoidosa were better than those of Chlorella vulgaris in both autotrophic and heterotrophic systems.The oil production rates of the two Chlorella species in the hetero⁃trophic system were 20.14%and 18.66%,respectively.When glucose was used as the carbon source,the growth of Chlorella pyre⁃noidosa was the best and the oil production rate was the highest,and the oil production rate was 26.40%.When the nitrogen source was urea,the maximum oil production rate of Chlorella pyrenoidosa was got,and was 32.70%.The highest oil production rate of Chlorella pyrenoidosa was 34.42%when the carbon-nitrogen ratio was 30∶1.The oil production rate of Chlorella pyrenoidosa under sulfur defi⁃ciency increased by 28.54 percentage points compared with the control.Under the interference of jasmonic acid with the concentration of 1.5 mg/L,the oil production rate of Chlorella pyrenoidosa was the highest,which was 40.42%.The results indicated that the growth and oil production rate of Chlorella pyrenoidosa were better than those of Chlorella vulgaris;glucose was the best carbon source;urea was the best nitrogen source;the oil production rate was the highest when the carbon nitrogen ratio was 30∶1;sulfur deficiency had a greater effect on Chlorella pyrenoidosa;the oil production rate of Chlorella pyrenoidosa was the highest under the interference of jas⁃monic acid with a concentration of 1.5 mg/L.