CITES监管植物习志野叶绿体基因组结构特征及系统发育分析

Characterization and Phylogenetic Analysis of Chloroplast Genome Structure of CITES Appendix Species Tephrocactus geometricus

利用二代测序技术Illumina平台对CITES附录监管物种习志野(Tephrocactus geometricus)进行叶绿体全基因组测序与组装,并对其基因组结构特征及系统进化关系进行分析。结果表明,习志野叶绿体基因组具备典型的环状四分体结构,全长116183 bp,包含72个蛋白编码基因、6个rRNA基因和30个tRNA基因。习志野叶绿体基因组共编码16119个密码子,编码亮氨酸(Leu)的最多;共发现微卫星位点259个,其中最多的是单核苷酸重复序列186个。采用ML法构建系统进化树,习志野与巨人柱属的巨人柱(Carnegiea gigantea)亲缘关系最近。本研究为习志野的分子标记开发、系统发育关系研究、叶绿体转化技术、濒危机制的解析等提供了基础数据参考,同时也丰富了仙人掌科植物的叶绿体基因组遗传信息。

The high-throughput sequencing of illumina platform was used to sequence and assembled the whole chloroplast genome of CITES Appendix Species Tephrocactus geometricus,and its structural characteristics and phylogenetic relationship were analysed.The results showed that the chloroplast genome was a typical tetrad structure.The genome was found to be 116183 bp in length,with 72 protein coding genes,6 ribosomal RNA genes,and 30 transfer RNA genes.The chloroplast genome encoded a total of 16119 codons,with Leu being the most common.A total of 259 microsatellite sites were detected in the chloroplast genome,with the highest single nucleotide repeat sequence being 186.A phylogenetic tree was constructed using the ML,which showed that T.geometricus was closely related to the Carnegiea gigantea.This study provided a theoretical basis for the development of molecular markers,research on phylogenetic relationships,chloroplast transformation techniques and analysis of endangered mechanisms.At the same time,it also enriched the genetic information of the chloroplast genome of Cactaceae plants.