细胞间黏附分子-1基因多态性与缺血性心肌病易感性及预后的关联

Association of Intercellular Adhesion Molecule-1 Gene Polymorphisms with Susceptibility and Prognosis of Ischemic Cardiomyopathy

目的探讨细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)基因多态性与缺血性心肌病(ischemic cardiomyopathy,ICM)易感性及预后的关系。方法选取因ICM入院的患者64例为ICM组,另选取健康体检者138例为对照组,采集两组血液标本并提取DNA。应用SNaPshot基因分型技术检测ICAM-1基因rs12462944、rs281430、rs281434、rs3093030、rs5491、rs923366共6个位点单核苷酸多态性,分析其与ICM易感性及预后的相关性。结果ICM组rs5491位点变异基因型AT频率高于对照组(P<0.05),等位基因T频率高于对照组(P<0.05),显性遗传模型中,rs281434位点变异基因型(AG+GG)频率高于对照组(P<0.05)。多因素Logistic回归分析结果显示,rs5491位点AT型携带者ICM风险是AA型的3.428倍(95%CI:1.152~7.747)。多因素COX回归分析结果显示,rs5491位点AT基因型是ICM心源性死亡的危险因素(HR=5.075,P<0.05),Kaplan-Meier生存曲线分析结果显示,AT基因型ICM患者较AA基因型3年内死亡风险更高(P<0.05)。结论ICAM-1rs5491基因型的变异提高了ICM的患病风险,AT型ICM患者较AA型预后较差。ICAM-1rs281434多态性可能与ICM易感性相关。

Objective To investigate the relationship between intercellular adhesion molecule-1(ICAM-1)gene polymorphism and susceptibility and prognosis of ischemic cardiomyopathy(ICM).Methods Sixty-four patients admitted for ICM were selected as the ICM group,and 138healthy subjects were selected as the control group.Blood specimens were collected and DNA was extracted from both groups.SNaPshot genotyping technique was applied to detect the SNP of ICAM-1 gene in six loci:rs12462944,rs281430,rs281434,rs3093030,rs5491,rs923366,and to analyze their correlation with the susceptibility and prognosis of ICM.Results The frequency of the rs5491 locus variant genotype AT was higher in the ICM group than that in the control group(P<0.05),the frequency of the allele T was higher than in the control group(P<0.05);in the dominant inheritance model and the frequency of the rs281434 locus variant genotype(AG+GG)was higher than that in the control group(P<0.05).Multivariate Logistic regression results showed that the risk of ICM in carriers of the AT type at locus rs5491 was 3.428 times higher than that of the AA type(95%CI:1.152-7.747).Multi-factorial COX regression results showed that the rs5491 locus AT genotype was a risk factor for cardiac death in ICM(HR=5.075,P<0.05),and Kaplan-Meier survival curves analysis showed that patients with ICM with the AT genotype had a higher risk of death at 3 years compared to the AA genotype(P<0.05).Conclusion Variants in ICAM-1rs5491 genotype increase the risk of ICM and patients with AT type ICM have a worse prognosis than AA type.The ICAM-1rs281434 polymorphism may be associated with susceptibility to ICM.